Journal Article

CD1a-Expressing Monocytes as Mediators of Inflammation in Ulcerative Colitis

Omar Al-amodi, Henrika Jodeleit, Florian Beigel, Eckhard Wolf, Matthias Siebeck and Roswitha Gropp

in Inflammatory Bowel Diseases

Volume 24, issue 6, pages 1225-1236
Published in print May 2018 | ISSN: 1078-0998
Published online May 2018 | e-ISSN: 1536-4844 | DOI: https://dx.doi.org/10.1093/ibd/izy073
CD1a-Expressing Monocytes as Mediators of Inflammation in Ulcerative Colitis

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Abstract

Background

CD1a-expressing CD14+ monocytes have been identified as inducers of autoreactive T cells. In this study, the link between inflammatory and metabolic signals and CD1a-expressing monocytes in vitro and in vivo was examined, and CD1a was evaluated as a potential therapeutic target for treatment of ulcerative colitis (UC).

Methods

Peripheral blood mononuclear cells (PBMCs) from UC patients and non-UC donors were incubated with phosphatidylcholine (PC) for 2 and 7 days and subjected to flow cytometric analysis. Triacylglycerol (TAG) and cholesterol levels and frequencies of CD14+ CD1a+ monocytes were determined in a mouse model of UC that is based on NOD/scid IL2Rγnull mice reconstituted with PBMCs from UC patients (NSG-UC). NSG-UC mice were treated with anti-CD1a antibodies. Response to treatment was determined by clinical and histological scores, flow cytometric analysis of human leucocytes from the spleen and colon, and expression levels of TGFß1, HGF, IFNγ, and TARC.

Results

Incubation of PBMCs with PC resulted in an increase of the frequency of CD1a+ CD14+ monocytes at the expense of CCR2-, CD86-, and TSLPR-expressing CD14+ monocytes. CD1a+ CD14+ monocytes induced the activation of CD4+ T cells and differentiation of Th cells. In vivo, TAG and cholesterol levels increased upon inflammation and correlated positively with CD14+ CD1a+ monocytes. NSG-UC mice benefitted from treatment with anti-CD1a antibodies, as indicated by a reduced histological score and reduced frequencies of CD1a+ CD14+ monocytes in the colon and spleen of mice.

Conclusion

CD1a-expressing monocytes might act as sensors and mediators of inflammation in UC. Mice benefitted from treatment with anti-CD1a antibodies.

Keywords: ulcerative colitis; CD1a; macrophage; monocyte; metabolic; inflammation

Journal Article.  6453 words.  Illustrated.

Subjects: Medicine and Health ; Clinical Medicine ; Gastroenterology ; Patient Education and Information ; Gastro-intestinal and Colorectal Surgery

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