Journal Article

IS26-mediated formation of a virulence and resistance plasmid in Salmonella Enteritidis

Marcus Ho-yin Wong, Edward Wai-chi Chan and Sheng Chen

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 72, issue 10, pages 2750-2754
Published in print October 2017 | ISSN: 0305-7453
Published online July 2017 | e-ISSN: 1460-2091 | DOI: https://dx.doi.org/10.1093/jac/dkx238
IS26-mediated formation of a virulence and resistance plasmid in Salmonella Enteritidis

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Abstract

Objectives

To characterize a novel virulence–resistance plasmid pSE380T carried by a Salmonella enterica serotype Enteritidis clinical strain SE380.

Methods

The plasmid pSE380T was conjugated to Escherichia coli strain J53 and sequenced by PacBio RSII, followed by subsequent annotation and genetic analysis.

Results

Sequence analysis of this plasmid revealed that the entire Salmonella Enteritidis-specific virulence plasmid, pSEN, had been incorporated into an IncHI2 MDR plasmid, which comprises the cephalosporin and fosfomycin resistance determinants blaCTX-M-14 and fosA3. Based on BLAST analysis and scrutiny of insertion footprints, the insertion event was found to involve a replicative transposition process mediated by IS26, an IS element frequently detected in various resistance plasmids. The resulting pSE380T plasmid also comprises backbone elements of IncHI2 and IncFIA plasmids, producing a rare fusion product that simultaneously encodes functional features of both, i.e. virulence, resistance and high transmissibility.

Conclusions

This is a novel hybrid plasmid mediating MDR and virulence from a clinical Salmonella Enteritidis strain. This plasmid is likely to be transmissible amongst various serotypes of Salmonella and other Enterobacteriaceae species, rendering a wide range of bacterial pathogens resistant to cephalosporins and fosfomycin, and further enhancing their virulence potential. It will be important to monitor the spread and further evolution of this plasmid among the Enterobacteriaceae strains.

Journal Article.  2174 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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