Journal Article

NMR and biophysical elucidation of structural effects on extra N-terminal methionine residue of recombinant amphibian RNases from Rana catesbeiana

Chun-Hua Hsu, Yun-Ru Pan, You-Di Liao, Shih-Hsiung Wu and Chinpan Chen

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 148, issue 2, pages 209-215
Published in print August 2010 | ISSN: 0021-924X
Published online June 2010 | e-ISSN: 1756-2651 | DOI: https://dx.doi.org/10.1093/jb/mvq058
NMR and biophysical elucidation of structural effects on extra N-terminal methionine residue of recombinant amphibian RNases from Rana catesbeiana

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The stability, structures and steric hindrances of recombinant RNases 2 and 4 expressed in bacteria were studied by circular dichroism (CD) and NMR techniques, and the results were compared with those of their authentic RNases extracted from oocytes of Rana catesbeiana. Although the overall structures of the recombinant and authentic proteins are almost identical, the extra N-terminal Met residue of the recombinant protein remarkably affects catalytic activity and stability. NMR chemical shift comparison of recombinant RNases and the authentic proteins indicated that the structural differences are mainly confined to the N-terminal helical and S2 anti-parallel β-sheet regions. Significant shift changes for the residues located on the S2 region indicate that the major influences on the structure around the N terminus is due to the loss of the hydrogen bond between Pyr1 and Val95(96) in recombinant RNases 2 and 4. We concluded the apparent steric hindrances of the extra Met to the binding pocket. As well, the affected conformational changes of active residues are attributed to the reduced activities of recombinant RNases. The structural integrity exerted by the N-terminal Pyr1 residue may be crucial for amphibian RNases and the greatest structural differences occur on the network of the Pyr1 residue and S2 β-sheet region.

Keywords: N-terminal extra methionine; NMR; protein stability; pyroglutamate; ribonuclease

Journal Article.  4288 words.  Illustrated.

Subjects: Biochemistry

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