Journal Article

Alpha-tocopherol succinate- and AMD3100-mobilized progenitors mitigate radiation combined injury in mice

Vijay K. Singh, Stephen Y. Wise, Oluseyi O. Fatanmi, Lindsay A. Beattie, Elizabeth J. Ducey and Thomas M. Seed

in Journal of Radiation Research

Published on behalf of Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology

Volume 55, issue 1, pages 41-53
Published in print January 2014 | ISSN: 0449-3060
Published online June 2013 | e-ISSN: 1349-9157 | DOI: https://dx.doi.org/10.1093/jrr/rrt088
Alpha-tocopherol succinate- and AMD3100-mobilized progenitors mitigate radiation combined injury in mice

More Like This

Show all results sharing these subjects:

  • Radiology
  • Nuclear Chemistry, Photochemistry, and Radiation

GO

Show Summary Details

Preview

The purpose of this study was to elucidate the role of alpha-tocopherol succinate (TS)- and AMD3100-mobilized progenitors in mitigating combined injury associated with acute radiation exposure in combination with secondary physical wounding. CD2F1 mice were exposed to high doses of cobalt-60 gamma-radiation and then transfused intravenously with 5 million peripheral blood mononuclear cells (PBMCs) from TS- and AMD3100-injected mice after irradiation. Within 1 h after irradiation, mice were exposed to secondary wounding. Mice were observed for 30 d after irradiation and cytokine analysis was conducted by multiplex Luminex assay at various time-points after irradiation and wounding. Our results initially demonstrated that transfusion of TS-mobilized progenitors from normal mice enhanced survival of acutely irradiated mice exposed 24 h prior to transfusion to supralethal doses (11.5–12.5 Gy) of 60Co gamma-radiation. Subsequently, comparable transfusions of TS-mobilized progenitors were shown to significantly mitigate severe combined injuries in acutely irradiated mice. TS administered 24 h before irradiation was able to protect mice against combined injury as well. Cytokine results demonstrated that wounding modulates irradiation-induced cytokines. This study further supports the conclusion that the infusion of TS-mobilized progenitor-containing PBMCs acts as a bridging therapy in radiation-combined-injury mice. We suggest that this novel bridging therapeutic approach involving the infusion of TS-mobilized hematopoietic progenitors following acute radiation exposure or combined injury might be applicable to humans.

Keywords: cytokines; gamma-radiation; hematopoietic progenitor cells; mice; transfusion

Journal Article.  6882 words.  Illustrated.

Subjects: Radiology ; Nuclear Chemistry, Photochemistry, and Radiation

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content. subscribe or login to access all content.