Journal Article

Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture

Xiaopeng Guo, Miaomiao Zhang, Yue Gao, Wenjian Li and Dong Lu

in Journal of Radiation Research

Volume 59, issue 6 Published in print November 2018 | ISSN: 0449-3060
Published online August 2018 | e-ISSN: 1349-9157 | DOI: https://dx.doi.org/10.1093/jrr/rry070
Determining survival fractions of Saccharomyces cerevisiae in response to ionizing radiation in liquid culture

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  • Clinical Genetics
  • Molecular Biology and Genetics
  • Epidemiology
  • Radiology
  • Nuclear Chemistry, Photochemistry, and Radiation

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Abstract

Saccharomyces cerevisiae survival fractions (SFs) in response to X-ray radiation were determined by two new methods: an OD600-based method and a 96-well method. For the OD600-based method, cells were exposed to various X-ray doses and inoculated into fresh medium: a lower biomass accumulated, indicating fewer surviving cells within the investigated dose range (0–100 Gy). For the 96-well method, diluent containing ~0–100 cells was equally divided into 96 droplets and respectively inoculated into 96 wells containing 200 μl of broth: fewer wells without S. cerevisiae clones indicated more surviving cells after 48 h of incubation. Corresponding quantitative systems were established. Both methods were sensitive and reliable. The OD600-based method is simple and fast, and the 96-well method simplifies the counting process. SF estimates by the OD600-based method were lower than those by 96-well methods owing to cell cycle arrest. In addition, comparisons of newly proposed and plate-counting methods indicated a higher rate of repair in S. cerevisiae in liquid culture than on agar.

Keywords: survival fractions; Saccharomyces cerevisiae; X-ray radiation; OD600; 96-well plate

Journal Article.  1954 words.  Illustrated.

Subjects: Clinical Genetics ; Molecular Biology and Genetics ; Epidemiology ; Radiology ; Nuclear Chemistry, Photochemistry, and Radiation

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