Journal Article

Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea

Adrian Radu Craciun, Mikael Courbot, Fabienne Bourgis, Pietrino Salis, Pierre Saumitou-Laprade and Nathalie Verbruggen

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 57, issue 12, pages 2967-2983
Published in print September 2006 | ISSN: 0022-0957
Published online August 2006 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erl062
Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea

More Like This

Show all results sharing this subject:

  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri. In order to identify genes potentially implicated in Cd tolerance, a backcross (BC1) segregating population was produced from crosses between A. halleri ssp. halleri and its closest non-tolerant relative A. lyrata ssp. petraea. The most sensitive and tolerant genotypes of the BC1 were analysed on a transcriptome-wide scale by cDNA-amplified fragment length polymorphism (AFLP). A hundred and thirty-four genes expressed more in the root of tolerant genotypes than in sensitive genotypes were identified. Most of the identified genes showed no regulation in their expression when exposed to Cd in a hydroponic culture medium and belonged to diverse functional classes, including reactive oxygen species (ROS) detoxification, cellular repair, metal sequestration, water transport, signal transduction, transcription regulation, and protein degradation, which are discussed.

Keywords: Arabidopsis halleri; cadmium; cDNA-AFLP; heavy metal tolerance; hyperaccumulator

Journal Article.  8613 words.  Illustrated.

Subjects: Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content. subscribe or purchase to access all content.