Journal Article

Proteome and metabolome profiling of cytokinin action in <i>Arabidopsis</i> identifying both distinct and similar responses to cytokinin down- and up-regulation

Martin Černý, Alena Kuklová, Wolfgang Hoehenwarter, Lena Fragner, Ondřej Novák, Gabriela Rotková, Petr L. Jedelský, Kateřina Žáková, Mária Šmehilová, Miroslav Strnad, Wolfram Weckwerth and Břetislav Brzobohatý

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 64, issue 14, pages 4193-4206
Published in print November 2013 | ISSN: 0022-0957
Published online September 2013 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/ert227

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In plants, numerous developmental processes are controlled by cytokinin (CK) levels and their ratios to levels of other hormones. While molecular mechanisms underlying the regulatory roles of CKs have been intensely researched, proteomic and metabolomic responses to CK deficiency are unknown. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S>GR>HvCKX2) and agrobacterial isopentenyl transferase (CaMV35S>GR>ipt) constructs were profiled to elucidate proteome- and metabolome-wide responses to down- and up-regulation of CK levels, respectively. Proteome profiling identified >1100 proteins, 155 of which responded to HvCKX2 and/or ipt activation, mostly involved in growth, development, and/or hormone and light signalling. The metabolome profiling covered 79 metabolites, 33 of which responded to HvCKX2 and/or ipt activation, mostly amino acids, carbohydrates, and organic acids. Comparison of the data sets obtained from activated CaMV35S>GR>HvCKX2 and CaMV35S>GR>ipt plants revealed unexpectedly extensive overlaps. Integration of the proteomic and metabolomic data sets revealed: (i) novel components of molecular circuits involved in CK action (e.g. ribosomal proteins); (ii) previously unrecognized links to redox regulation and stress hormone signalling networks; and (iii) CK content markers. The striking overlaps in profiles observed in CK-deficient and CK-overproducing seedlings might explain surprising previously reported similarities between plants with down- and up-regulated CK levels.

Keywords: Arabidopsis thaliana; cytokinin; cytokinin oxidase/dehydrogenase; isopentenyl transferase; metabolome; proteome.

Journal Article.  9886 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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