Journal Article

Rodent UV-Sensitive Mutant Cell Lines in Complementation Groups 6–10 Have Normal General Excision Repair Activity

Joyce T. Reardon, Larry H. Thompson and Aziz Sancar

in Nucleic Acids Research

Volume 25, issue 5, pages 1015-1021
Published in print March 1997 | ISSN: 0305-1048
Published online March 1997 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/25.5.1015
Rodent UV-Sensitive Mutant Cell Lines in Complementation Groups 6–10 Have Normal General Excision Repair Activity

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Mammalian nucleotide excision repair is the primary enzymatic pathway for removing bulky lesions from DNA. The repair reaction involves three main steps: (i) dual incisions on both sides of the lesion; (ii) excision of the damaged base in an oligonucleotide 24–31 nt in length; (iii) filling in of the post-excision gap and ligation. We have developed assays that probe the individual steps of the reaction. Using these methods (assays for incision, excision and repair patch synthesis), we demonstrate that the mammalian excision nuclease system removes bulky lesions by incising mainly at the...

Journal Article.  4239 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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