Journal Article

Mapping analysis of the Xylella fastidiosa genome

Marcus Frohme, Anamaria A. Camargo, Steffen Heber, Claudia Czink, Andrew J. D. Simpson, Jörg D. Hoheisel and Anete Pereira de Souza

in Nucleic Acids Research

Volume 28, issue 16, pages 3100-3104
Published in print August 2000 | ISSN: 0305-1048
Published online August 2000 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/28.16.3100
Mapping analysis of the Xylella fastidiosa genome

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A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity of the cosmid map, and thus the eventual sequence, could be confirmed by this process. A subset of the eventual clone coverage was distributed to the Brazilian X.fastidiosa sequencing network. Data from this effort confirmed more quantitatively initial results from the hybridisation mapping that the redundancy of clone coverage ranged between 0 and 45-fold across the genome, while the average was 15-fold by experimental design. Reasons for this not unexpected fluctuation and the actual gaps are being discussed, as is the use of this effect for functional studies.

Journal Article.  3252 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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