Journal Article

Repair of DNA-protein crosslink damage: Coordinated actions of nucleotide excision repair and homologous recombination

Hiroshi Ide, Toshiaki Nakano, Amir M.H. Salem, Hiroaki Terato, Seung Pil Pack and Keisuke Makino

in Nucleic Acids Symposium Series

Volume 52, issue 1, pages 57-58
Published in print September 2008 | ISSN: 0261-3166
Published online September 2008 | e-ISSN: 1746-8272 | DOI: https://dx.doi.org/10.1093/nass/nrn029
Repair of DNA-protein crosslink damage: Coordinated actions of nucleotide excision repair and homologous recombination

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DNA-protein crosslinks (DPCs) are extremely bulky DNA lesions, and steric hindrance imposed by covalently trapped proteins would hamper the transaction of DNA such as replication, transcription, and repair. However, it has been largely elusive how cells mitigate the genotoxic effect of DPCs. We have recently shown that nucleotide excision repair (NER) and homologous recombination (HR) differentially contribute to the repair of DPCs in E. coli cells. Several lines of genetic and biochemical evidence indicate that NER repairs DPCs with crosslinked proteins (CLPs) of sizes less than 12–14 kDa, whereas DPCs with oversized CLPs are processed exclusively by RecBCD-dependent HR. The present result shows that cells use the coordinated actions of NER and HR to deal with unusually bulky DNA lesions like DPCs.

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Subjects: Chemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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