Journal Article

Accelerating phage-display library selection by reversible and site-specific biotinylation

Akiko Koide, John Wojcik, Ryan N. Gilbreth, Annett Reichel, Jacob Piehler and Shohei Koide

in Protein Engineering, Design and Selection

Volume 22, issue 11, pages 685-690
Published in print November 2009 | ISSN: 1741-0126
Published online September 2009 | e-ISSN: 1741-0134 | DOI: https://dx.doi.org/10.1093/protein/gzp053
Accelerating phage-display library selection by reversible and site-specific biotinylation

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Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of existing strategies. Our method is based on the use of a polyhistidine-tagged (His-tagged) target molecule and BTtris-NTA, a high-affinity capture reagent for His-tags that also contains a biotin moiety. BTtris-NTA provides a stable and reversible linkage between a His-tag and a streptavidin-coated solid support. Because His-tags are the de facto standard for recombinant protein purification, this method dramatically simplifies target preparation for phage display library sorting. Here, we demonstrate the utility of this method by selecting high-affinity binding proteins based on the fibronectin type III (FN3) scaffold to two His-tagged protein targets, yeast small ubiquitin-like modifier and maltose-binding protein. Notably, a significant number of FN3 clones binding either targets selected using the new immobilization method exhibited only very weak binding when the same target was immobilized by coating on a polystyrene surface. This suggests that the His-tag-mediated immobilization exposes epitopes that are masked by commonly used passive adsorption methods. Together, these results establish a method with the potential to streamline and enhance many binding-protein engineering experiments.

Keywords: directed evolution; high-throughput selection; monobodies; synthetic binding proteins

Journal Article.  4184 words.  Illustrated.

Subjects: Proteins

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