Journal Article

Bovine serum albumin adsorption onto functionalized polystyrene lattices: A theoretical modeling approach and error analysis

Manel Beragoui, Chadlia Aguir, Mohamed Khalfaoui, Eduardo Enciso, Maria José Torralvo, Laurent Duclaux, Laurence Reinert, Marylène Vayer and Abdelmottaleb Ben Lamine

in Progress of Theoretical and Experimental Physics

Published on behalf of The Physical Society of Japan

Volume 2015, issue 3 Published in print March 2015 |
Published online March 2015 | e-ISSN: 2050-3911 | DOI:

More Like This

Show all results sharing these subjects:

  • Physics
  • Biological and Medical Physics


Show Summary Details


The present work involves the study of bovine serum albumin adsorption onto five functionalized polystyrene lattices. The adsorption measurements have been carried out using a quartz crystal microbalance. Poly(styrene-co-itaconic acid) was found to be an effective adsorbent for bovine serum albumin molecule adsorption. The experimental isotherm data were analyzed using theoretical models based on a statistical physics approach, namely monolayer, double layer with two successive energy levels, finite multilayer, and modified Brunauer–Emmet–Teller. The equilibrium data were then analyzed using five different non-linear error analysis methods and it was found that the finite multilayer model best describes the protein adsorption data. Surface characteristics, i.e., surface charge density and number density of surface carboxyl groups, were used to investigate their effect on the adsorption capacity. The combination of the results obtained from the number of adsorbed layers, the number of adsorbed molecules per site, and the thickness of the adsorbed bovine serum albumin layer allows us to predict that the adsorption of this protein molecule can also be distinguished by monolayer or multilayer adsorption with end-on, side-on, and overlap conformations. The magnitudes of the calculated adsorption energy indicate that bovine serum albumin molecules are physisorbed onto the adsorbent lattices.

Keywords: J36; J41; J50

Journal Article.  8165 words.  Illustrated.

Subjects: Physics ; Biological and Medical Physics

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content. subscribe or login to access all content.