Journal Article

Identification of a Transcriptional Fingerprint of Estrogen Exposure in Rainbow Trout Liver

Abby D. Benninghoff and David E. Williams

in Toxicological Sciences

Volume 101, issue 1, pages 65-80
Published in print January 2008 | ISSN: 1096-6080
Published online September 2007 | e-ISSN: 1096-0929 | DOI:
Identification of a Transcriptional Fingerprint of Estrogen Exposure in Rainbow Trout Liver

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The goal of this study was to identify a set of hepatic genes regulated by ligand-dependent activation of the estrogen receptor in juvenile rainbow trout (Oncorhynchus mykiss). A custom rainbow trout oligo DNA microarray, which contains probes targeting approximately 1450 genes relevant to carcinogenesis, toxicology, endocrinology, and stress physiology was utilized to identify transcriptional fingerprints of in vivo dietary exposure to 17β-estradiol (E2), tamoxifen (TAM), estradiol + tamoxifen (E2 + TAM), diethylstilbestrol (DES), dehydroepiandrosterone (DHEA), dihydrotestosterone (DHT), and cortisol (CORT). Estrogen exposure altered the expression of up to 49 genes involved in reproduction, immune response, cell growth, transcriptional regulation, protein synthesis and modification, drug metabolism, redox regulation, and signal transduction. E2, DES, and DHEA regulated 18 genes in common, mostly those associated with vitellogenesis, cell proliferation, and signal transduction. Interestingly, DHEA uniquely regulated several complement component genes of importance to immune response. While the effect of TAM on E2-induced changes in gene expression was mostly antagonistic, TAM alone increased expression of VTG1 and other genes associated with egg development and immune response. Few genes responded to CORT treatment, and DHT significantly altered expression of only one gene targeted by the OSUrbt array. Hierarchical cluster and principal components analyses revealed distinct patterns of gene expression corresponding to estrogens and non-estrogens, though unique patterns could also be detected for individual chemicals. A set of estrogen-responsive genes has been identified that can serve as a biomarker of environmental exposure to xenoestrogens.

Keywords: estrogen; toxicogenomics; transcription profile; dehydroepiandrosterone; tamoxifen; genomics

Journal Article.  8826 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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