Journal Article

Immunodiagnoses of Community-acquired Pneumonia in Childhood

Henry I. Z. Requejo, Maria Luiza L. S. Guerre, Marlina Dos Santos and Ana Maria Cocozza

in Journal of Tropical Pediatrics

Volume 43, issue 4, pages 208-212
Published in print August 1997 | ISSN: 0142-6338
Published online August 1997 | e-ISSN: 1465-3664 | DOI: https://dx.doi.org/10.1093/tropej/43.4.208
Immunodiagnoses of Community-acquired Pneumonia in Childhood

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SUMMARY

A diagnosis of bacterial pneumonia requires isolation of the pathogen from blood, lung or tracheal aspirate; however, cultures of blood and pleural fluid samples are usually insensitive. Thus, in the majority of patients the etiology is rarely determined. A total of 840 pleural fluid effusion samples from children with clinical and laboratory diagnoses of acute bacterial pneumonia were evaluated by Dot-ELISA. This method was standardized in order to detect polysaccharide capsular bacterial antigen in pleural fluid samples previously treated with 0.1 M EDTA and dotted on nitrocellulose membrane strips. Pneumococcal omniserum and H. influenzae type b antiserum diluted 1:200 were employed for detection of S. pneumoniae and H. influenzae type b antigens, respectively. Dot-ELISA showed relative indices of 0.913 for sensitivity and 0.552 for specificity, and a total positivity of 75.6 per cent, being 53.21 per cent for S. pneumoniae and of 22.38 per cent for H. influenzae.

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Subjects: Paediatrics ; Antitrust Issues and Policies

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