Journal Article

Development of neutralising human recombinant antibodies to pertussis toxin

Peter Williamson and Ruth Matthews

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 23, issue 4, pages 313-319
Published in print April 1999 | ISSN: 0928-8244
Published online January 2006 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1111/j.1574-695X.1999.tb01253.x
Development of neutralising human recombinant antibodies to pertussis toxin

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Abstract

A phage antibody display library of single chain Fv (scFv) was derived from the peripheral blood of two patients recently recovered from pertussis infection. Ten scFv, differentiated by DNA fingerprinting, were isolated by panning the library against pertussis toxin. One scFv (type 1) accounted for 33% of clones after panning. Six of the panned scFv bound to pertussis toxin. The ability of the scFv to neutralise pertussis toxin was assessed using the Chinese hamster ovary cell assay. The predominant scFv (type I) and two others (types IV and VIII) were able to neutralise the pertussis toxin.

Keywords: Pertussis toxin; Phage display; Human recombinant antibody

Journal Article.  3480 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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