Journal Article

Recombinant BCG coexpressing Ag85B, ESAT-6 and mouse-IFN-γ confers effective protection against Mycobacterium tuberculosis in C57BL/6 mice

Ying Xu, Bingdong Zhu, Qingzhong Wang, Jiazhen Chen, Yaqing Qie, Jiuling Wang, Hongyan Wang, Baolin Wang and Honghai Wang

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 51, issue 3, pages 480-487
Published in print December 2007 | ISSN: 0928-8244
Published online October 2007 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1111/j.1574-695X.2007.00322.x
Recombinant BCG coexpressing Ag85B, ESAT-6 and mouse-IFN-γ confers effective protection against Mycobacterium tuberculosis in C57BL/6 mice

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Abstract

In this study, the protective efficacy of a novel recombinant bacille Calmette Géurin (BCG) strain (rBCG-AEI) expressing fusion protein the antigen 85B (Ag85B)- the 6-kDa early secreted antigen target (ESAT-6)-IFN-γ against Mycobacterium tuberculosis H37Rv in mice was evaluated. The immunogenicity study showed that rBCG-AEI could induce higher specific antibody titers and significantly increase cellular immune response than BCG, or rBCG-A strain (expressing Ag85B), or rBCG-AE strain (expressing fusion protein Ag85B-ESAT-6). The protective experiment demonstrated that rBCG-AEI could confer similar or even better protective efficacy against M. tuberculosis infection compared with others in organ bacterial loads, lung histopathology and net weight gain or loss. The results suggested that rBCG-AEI is a potential candidate for further study.

Keywords: Mycobacterium tuberculosis; rBCG; IFN-γ

Journal Article.  4264 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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