Journal Article

Association of TNF-α Gene Promoter Polymorphisms With Susceptibility of Cervical Cancer in Southwest China

Fengqiong Zuo, Weibo Liang, Yunwei Ouyang, Wanyi Li, Meili Lv, Guoyu Wang, Mingpu Ding, Baoning Wang, Suhua Zhao, Jin Liu, Zhonghua Jiang and Mingyuan Li

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 42, issue 5, pages 287-290
Published in print May 2011 | ISSN: 0007-5027
Published online October 2015 | e-ISSN: 1943-7730 | DOI: https://dx.doi.org/10.1309/LM532DSPDUXIRJVN
Association of TNF-α Gene Promoter Polymorphisms With Susceptibility of Cervical Cancer in Southwest China

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  • Clinical Cytogenetics and Molecular Genetics
  • Molecular and Cell Biology
  • Molecular Biology and Genetics

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Objective:

Variations in the tumor necrosis factor-alpha (TNF-α) gene may lead to changes in the level of TNF-α associated with the susceptibility to cancer. This study is designed to determine the association of TNF-α promoter polymorphisms with the susceptibility of cervical cancer among women living in Southwest China.

Methods:

Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing, we analyzed the genotype and allele distributions of 4 single-nucleotide polymorphisms (SNP) of TNF-α gene in 239 cervical cancer patients and 110 controls.

Results:

Compared to controls, cervical cancer patients show a significant increase in the frequency of GA genotype at −308 G/A, and a significantly decreased frequency of the CT genotype. A significant decrease in the frequency of the T allele at −857 C/T (P<0.05) was also found. No significant differences of SNP genotype and allele at TNF-α-863 C/A and −238 G/A were observed between the 2 groups.

Conclusion:

An SNP at TNF-α −857 C/T and −308 G/A, but not −863 C/A or −238 G/A, were significantly associated with an increased risk of cervical cancer in the studied population.

Keywords: cervical cancer; tumor necrosis factor; allele; genotype; single nucleotide polymorphisms

Journal Article.  2848 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics ; Molecular and Cell Biology ; Molecular Biology and Genetics

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