Journal Article

Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method

Aleksandra Vancevska and Aleksandra Nikolic

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 44, issue 2, pages 125-128
Published in print May 2013 | ISSN: 0007-5027
Published online September 2015 | e-ISSN: 1943-7730 | DOI: https://dx.doi.org/10.1309/LMD9INNMFDO5XGIW
Assessment of Deoxyribonuclease Activity in Biological Samples by a Fluorescence Detection-Based Method

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  • Clinical Cytogenetics and Molecular Genetics
  • Molecular and Cell Biology
  • Molecular Biology and Genetics

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Objective:

To develop a speedy, sensitive, low-cost fluorescence detection assay for assessing deoxyribonuclease activity in biological samples.

Methods:

Deoxyribonuclease activity was measured in serum samples of 15 patients with lung cancer and 15 healthy individuals. The assay is based on hydrolysis of fluorescently labeled DNA into fragments that are subsequently measured by capillary electrophoresis.

Results:

Mean (SD) reduction in signal intensity compared with the negative control reaction was 0.04 (0.09) for the group of patients with lung cancer and 0.01 (0.07) for the control group. The difference in signal intensity between the 2 groups was not statistically significant.

Conclusion:

The main advantages of the fluorescence detection assay are its high sensitivity, the short time required for it to obtain results, and its low cost. The method provides a useful alternative to the conventional enzyme-linked immunosorbent assay (ELISA) and can potentially be applied to a variety of studies, especially when assessment of deoxyribonuclease activity in large sample series is required.

Keywords: deoxyribonuclease; fluorescence; capillary electrophoresis; serum

Journal Article.  2052 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics ; Molecular and Cell Biology ; Molecular Biology and Genetics

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