Journal Article

Quantitative Real-Time PCR Analysis of Intestinal Regular Fungal Species in Fecal Samples From Patients With Chronic Hepatitis B Virus Infection

Renyong Guo, Zhenjing Chen, Nan Chen and Yu Chen

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 41, issue 10, pages 591-596
Published in print October 2010 | ISSN: 0007-5027
Published online October 2015 | e-ISSN: 1943-7730 | DOI: https://dx.doi.org/10.1309/LMMC0WVZXD13PUJG
Quantitative Real-Time PCR Analysis of Intestinal Regular Fungal Species in Fecal Samples From Patients With Chronic Hepatitis B Virus Infection

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  • Clinical Cytogenetics and Molecular Genetics
  • Molecular and Cell Biology
  • Molecular Biology and Genetics

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Objective:

To clarify the intestinal fungal flora imbalance at varied stages of chronic Hepatitis B Virus infection.

Methods:

Real-time polymerase chain reaction (PCR) was used to investigate the intestinal Candida albicans, Candida parapsilosis, Candida tropicalis, Candida glabrata, Candida krusei, and Saccharomyces cerevisiae in HBV-liver cirrhosis (LC) patients, chronic hepatitis (CH) B patients, HBV carriers (HC), and healthy volunteers. The distribution of intestinal fungi in relation to the staging of chronic HBV infection was analyzed.

Results:

Target DNA copy number and prevalence value of intestinal regular fungal species were much higher in patients with chronic HBV infection than in healthy volunteers, especially in LC and CH. The most numerous fungal species in LC, CH, HC, and healthy volunteers was C. krusei, S. cerevisiae, S. cerevisiae, and C. krusei, respectively, while S. cerevisiae, C. krusei, C. albicans, and C. albicans were more frequently found in the groups described above, respectively.

Conclusion:

Proliferations of intestinal regular fungi existed in patients at varied stages of chronic HBV infection, and the degree of flora imbalance correlated with the severity of HBV infection.

Keywords: hepatitis B virus; fungi; intestine; real-time PCR

Journal Article.  4145 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics ; Molecular and Cell Biology ; Molecular Biology and Genetics

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