Journal Article

Real-Time Polymerase Chain Reaction Testing for Quantitative Evaluation of hOCT1 and MDR1 Expression in Patients with Chronic Myeloid Leukemia Resistant to Imatinib

Saeed Solali, Saeed Kaviani, Ali Akbar Movassaghpour and Mohammad Reza Aliparasti

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 44, issue 1, pages 13-19
Published in print February 2013 | ISSN: 0007-5027
Published online September 2015 | e-ISSN: 1943-7730 | DOI: https://dx.doi.org/10.1309/LMP1ECAE30JSVZEP
Real-Time Polymerase Chain Reaction Testing for Quantitative Evaluation of hOCT1 and MDR1 Expression in Patients with Chronic Myeloid Leukemia Resistant to Imatinib

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  • Haematology
  • Clinical Cytogenetics and Molecular Genetics
  • Molecular and Cell Biology
  • Molecular Biology and Genetics

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Objective

To study quantitative expression of the genes MDR1 and hOCT1 in 30 patients with chronic myeloid leukemia (CML) and 27 healthy subjects.

Methods

Total RNA was extracted from peripheral blood mononuclear cells. Complementary DNAs (cDNAs) were synthesized and realtime polymerase chain reaction (PCR) was performed. To evaluate the specificity of PCR products, melting curve analyses and gel electrophoresis were performed.

Results

Our study results revealed an increased expression of MDR1 in CML patients compared to healthy controls. Patients with disease in the accelerated phase (AP) and in blastic crisis (BC) displayed higher expression of MDR1 compared with patients whose disease was in the chronic phase (CP).However, there was no significant difference in hOCT1-gene expression among CML patients and healthy controls.

Conclusion

Increased expression of MDR1 can reduce the intracellular concentration of imatinib inside leukemic cells, resulting in the development of resistance to this drug. However, hOCT1 may play no significant role in the susceptibility of leukemic cells to imatinib in CML.

Keywords: MDR1; hOCT1; CML; imatinib; real-time PCR

Journal Article.  3547 words.  Illustrated.

Subjects: Haematology ; Clinical Cytogenetics and Molecular Genetics ; Molecular and Cell Biology ; Molecular Biology and Genetics

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