Journal Article

Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey

Ali Rezaei-Matehkolaei, Koichi Makimura, Sybren de Hoog, Mohammad Reza Shidfar, Farideh Zaini, Mohammadreza Eshraghian, Parvaneh Adimi Naghan and Hossein Mirhendi

in Medical Mycology

Published on behalf of International Society for Human and Animal Mycology

Volume 51, issue 2, pages 203-207
Published in print February 2013 | ISSN: 1369-3786
Published online February 2013 | e-ISSN: 1460-2709 | DOI: https://dx.doi.org/10.3109/13693786.2012.686124
Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey

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  • Mycology and Fungi
  • Infectious Diseases
  • Medical Toxicology
  • Veterinary Medicine
  • Environmental Science

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In the framework of a survey on dermatophytoses, 14,619 clinical specimens taken from outpatients with symptoms suggestive of tinea and referred to a Medical Mycology laboratory in Tehran, Iran, were analyzed by direct microscopy and culture. In total, 777 dermatophyte strains recovered in culture were randomly identified by a formerly established RFLP analysis method based on the rDNA ITS regions. For confirmation of species identification, 160 isolates representing the likely entire species spectrum were subjected to ITS-sequencing. Infection was confirmed in 5,175 collected samples (35.4%) by direct microscopy and/or culture. Tinea pedis was the most prevalent type of infection (43.4%), followed by tinea unguium (21.3%), tinea cruris (20.7%), tinea corporis (9.4%), tinea manuum (4.2%), tinea capitis (0.8%) and tinea faciei (0.2%). Trichophyton interdigitale was the most common isolate (40.5%) followed by T. rubrum (34.75%), Epidermophyton floccosum (15.6%), Microsporum canis (3.9%), T. tonsurans (3.5 %) and M. gypseum (0.5%). Other species included M. ferrugineum, T. erinacei, T. violaceum, T. schoenleinii, and a very rare species T. eriotrephon (each one 0.25%). The two strains of T. eriotrephon isolated from tinea manuum and tinea faciei are the second and third reported cases worldwide. Application of DNA-based methods is an important aid in monitoring trends in dermatophytosis in the community.

Keywords: dermatophytosis; molecular epidemiology; ITS-PCR-RFLP; Tehran

Journal Article.  2539 words.  Illustrated.

Subjects: Mycology and Fungi ; Infectious Diseases ; Medical Toxicology ; Veterinary Medicine ; Environmental Science

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