Journal Article

Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β

S. H. Lee, H. S. Lillehoj, M. S. Jeong, E. Del Cacho, J. B. Kim, H. R. Kim, W. Min, H. Y. Jeoung and D. J. An

in Poultry Science

Volume 93, issue 9, pages 2193-2198
Published in print September 2014 | ISSN: 0032-5791
Published online August 2014 | e-ISSN: 1525-3171 | DOI: https://dx.doi.org/10.3382/ps.2014-03947
Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β

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  • Zoology and Animal Sciences
  • Animal Pathology and Diseases
  • Animal Physiology
  • Ornithology
  • Veterinary Medicine

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Interleukin-1β proteins from chicken, duck, goose, turkey, and pigeon share 77 to 99% amino acid sequence similarity among themselves, and only 31 to 35% sequence similarity is shared between avian and mammalian IL-1β. There have been no antibodies that specifically detect avian IL-1β, and the current study was conducted to develop mouse monoclonal antibodies (mAb) against chicken IL-1β (chIL-1β) to further define its biochemical and immunological properties. In this study, 2 mouse mAb that are specific for chIL-1β were produced and characterized. Both mAb identified a 66.0 kDa recombinant chIL-1β protein expressed in Escherichia coli by Western blot analysis that corresponded to the expected molecular weight of a recombinant fusion protein containing the full-length 23.0 kDa chIL-1β protein and a 43.0 kDa maltose binding protein tag. Immunohistochemical analysis identified cells producing endogenous chIL-1β in the cecal tonsils, bursa of Fabricius, and spleen. Purified recombinant chIL-1β dose-dependently stimulated the proliferation and nitric oxide production by thymocytes, and both activities were inhibited by co-incubation with the 2 chIL-1β mAb described in this paper. These mAb will be important immune reagents for basic and applied poultry research of IL-1β in poultry.

Keywords: interleukin 1β; monoclonal antibody; chicken; thymus; proliferation

Journal Article.  3562 words.  Illustrated.

Subjects: Zoology and Animal Sciences ; Animal Pathology and Diseases ; Animal Physiology ; Ornithology ; Veterinary Medicine

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