A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure.
in Protein Engineering, Design and Selection
October 1998; p ublished online October 1998 .
Journal Article. Subjects: Proteins. 0 words.
In the crystal structure of uncomplexed native chymosin, the beta-hairpin at the active site, known as 'the flap', adopts a different conformation from that of other aspartic proteinases....
Go to » abstract
A 3D model of the δ opioid receptor and ligand-receptor complexes
in Protein Engineering, Design and Selection
July 1996 .
Journal Article. Subjects: Proteins. 0 words.
A model for the 3D structure of the transmembrane domain of the δ opioid receptor was predicted from the sequence divergence analysis of 42 sequences of G-protein coupled peptide hormone...
Go to » abstract
3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM
in Nucleic Acids Research
September 2016; p ublished online August 2016 .
Journal Article. Subjects: Molecular and Cell Biology; Genetics and Genomics; Research Methods in Life Sciences; Proteins; Molecular Biology and Genetics. 3716 words.
The Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device that enables contrast enhancement of biological samples. Here we have evaluated the potential of...
Go to » full text: open access
A33scFv–Green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting
in Protein Engineering, Design and Selection
December 2007; p ublished online November 2007 .
Journal Article. Subjects: Proteins. 4874 words.
Chemical conjugates of monoclonal antibodies with fluorophores or enzymes have long been used for diagnostic purposes and experimental therapeutic approaches. Recombinant technology allows...
Go to » abstract
Absolute conservation of residue 6 of immunoglobulin heavy chain variable regions of class IIA is required for correct folding.
in Protein Engineering, Design and Selection
December 1998; p ublished online December 1998 .
Journal Article. Subjects: Proteins. 0 words.
While studying the expression of single-chain antibodies (scFv) derived from several murine monoclonal antibodies, we found that residue 6 in Framework region 1 of the heavy chain variable...
Go to » abstract
An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei l-lactate dehydrogenase activity induced by a single amino acid substitution
in Protein Engineering, Design and Selection
January 2002; p ublished online January 2002 .
Journal Article. Subjects: Proteins. 5234 words.
Lactobacillus casei allosteric L-lactate dehydrogenase (l-LDH) absolutely requires fructose 1,6-bisphosphate [Fru(1,6)P2] for its catalytic activity under neutral conditions,...
Go to » abstract
Abstracts of the 8th Annual Meeting of the Protein Engineering Society of Japan
in Protein Engineering, Design and Selection
September 1996 .
Journal Article. Subjects: Proteins. 0 words.
Go to » abstract
Accelerating phage-display library selection by reversible and site-specific biotinylation
in Protein Engineering, Design and Selection
November 2009; p ublished online September 2009 .
Journal Article. Subjects: Proteins. 4184 words.
Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of...
Go to » abstract
Accurate ab initio prediction of NMR chemical shifts of nucleic acids and nucleic acids/protein complexes
in Nucleic Acids Research
December 2014; p ublished online November 2014 .
Journal Article. Subjects: Molecular and Cell Biology; Proteins; Molecular Biology and Genetics. 7813 words.
NMR chemical shift predictions based on empirical methods are nowadays indispensable tools during resonance assignment and 3D structure calculation of proteins. However, owing to the very...
Go to » full text: open access
Acetylcholinesterase engineering for detection of insecticide residues
in Protein Engineering, Design and Selection
January 2002; p ublished online January 2002 .
Journal Article. Subjects: Proteins. 3723 words.
To detect traces of insecticides in the environment using biosensors, we engineered Drosophila acetylcholinesterase (AChE) to increase its sensitivity and its rate of phosphorylation or...
Go to » abstract
Acknowledgements
in Protein Engineering, Design and Selection
December 1996 .
Journal Article. Subjects: Proteins. 0 words.
Go to » abstract
Activation of E350A mutant maltodextrin phosphorylase by exogenously added acetate
in Protein Engineering, Design and Selection
August 1996 .
Journal Article. Subjects: Proteins. 0 words.
Site-directed mutagenesis of E350 to alanine in Escherichia coli maltodextrin phosphorylase reduced both enzyme activity (100-fold) and apparent binding of the oligosaccharide substrate...
Go to » abstract
Activation of the low oxygen affinity-inducing potential of the Asn108(β)→Lys mutation of Hb-Presbyterian on intramolecular αα-fumaryl cross-bridging
in Protein Engineering, Design and Selection
May 2001; p ublished online May 2001 .
Journal Article. Subjects: Proteins. 5394 words.
The Asn108β→Lys mutation in hemoglobin (HbPresbyterian mutation) endows a low O2 affinity-inducing propensity to the protein. Introduction of a fumaryl cross-bridge between its two α99...
Go to » abstract
The active site of carboxypeptidase Taq possesses the active-site motif His–Glu–X–X-His of zinc-dependent endopeptidases and aminopeptidases
in Protein Engineering, Design and Selection
June 1996 .
Journal Article. Subjects: Proteins. 0 words.
Carboxypeptidase (CPase) Taq possesses the His–Glu–X–X–His sequence, which is the consensus sequence in the active site of zinc-dependent endopeptidases and amino-peptidases, at positions...
Go to » abstract
The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169
in Protein Engineering, Design and Selection
August 1996 .
Journal Article. Subjects: Proteins. 0 words.
Trichoderma reesei cellobiohydrolase II (CBHII) is an exoglucanase cleaving primarily cellobiose units from the non-reducing end of cellulose chains. The β-l,4 glycosidic bond...
Go to » abstract
Active-site residues are critical for the folding and stability of methylamine dehydrogenase
in Protein Engineering, Design and Selection
September 2001; p ublished online September 2001 .
Journal Article. Subjects: Proteins. 6180 words.
Site-directed mutagenesis was used to alter active-site residues of methylamine dehydrogenase (MADH) from Paracoccus denitrificans. Four residues of the β subunit of MADH which are in close...
Go to » abstract
Activity enhancement of CotA laccase by hydrophilic engineering, histidine tag optimization and static culture
in Protein Engineering, Design and Selection
January 2018; p ublished online December 2017 .
Journal Article. Subjects: Proteins. 2249 words.
Abstract
CotA protein from Bacillus subtilis is of laccase activity. The solubility of recombinant CotA is low, which hinders its application. In this study,...
Activity of linked HIV-1 proteinase dimers containing mutations in the active site region
in Protein Engineering, Design and Selection
November 1996 .
Journal Article. Subjects: Proteins. 0 words.
Mutations were introduced into the active site triplet (Asp–Thr–Gly) of one or both subunits of a linked dimer of human immunodeficiency virus type 1 proteinase. Mutation of Thr to Ser in...
Go to » abstract
Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures
in Protein Engineering, Design and Selection
May 2010; p ublished online February 2010 .
Journal Article. Subjects: Proteins. 5965 words.
Group II chaperonins exist in archaea and the eukaryotic cytosol, and mediate protein folding in an ATP-dependent manner. We have been studying the reaction mechanism of group II...
Go to » abstract
Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures
in Protein Engineering, Design and Selection
February 2001; p ublished online February 2001 .
Journal Article. Subjects: Proteins. 4698 words.
Random mutagenesis coupled with screening of the active enzyme at a low temperature was applied to isolate cold-adapted mutants of a thermophilic enzyme. Four mutant enzymes with enhanced...
Go to » abstract
