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A 2.3 A resolution structure of chymosin complexed with a reduced bond inhibitor shows that the active site beta-hairpin flap is rearranged when compared with the native crystal structure.

M R Groves, V Dhanaraj, M Badasso, P Nugent, J E Pitts, D J Hoover and T L Blundell.

in Protein Engineering, Design and Selection

October 1998; p ublished online October 1998 .

Journal Article. Subjects: Proteins. 0 words.

In the crystal structure of uncomplexed native chymosin, the beta-hairpin at the active site, known as 'the flap', adopts a different conformation from that of other aspartic proteinases....

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A 3D model of the δ opioid receptor and ligand-receptor complexes

Ibon Alkorta and Gilda H. Loew.

in Protein Engineering, Design and Selection

July 1996 .

Journal Article. Subjects: Proteins. 0 words.

A model for the 3D structure of the transmembrane domain of the δ opioid receptor was predicted from the sequence divergence analysis of 42 sequences of G-protein coupled peptide hormone...

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3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM

Eugene Y.D. Chua, Vinod K. Vogirala, Oviya Inian, Andrew S.W. Wong, Lars Nordenskiöld, Juergen M. Plitzko, Radostin Danev and Sara Sandin.

in Nucleic Acids Research

September 2016; p ublished online August 2016 .

Journal Article. Subjects: Molecular and Cell Biology; Genetics and Genomics; Research Methods in Life Sciences; Proteins; Molecular Biology and Genetics. 3716 words.

The Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device that enables contrast enhancement of biological samples. Here we have evaluated the potential of...

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A33scFv–Green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting

Ulf Petrausch, Jens Dernedde, Vânia Coelho, Hossein Panjideh, Dietmar Frey, Hendrik Fuchs, Eckhard Thiel and P. Markus Deckert.

in Protein Engineering, Design and Selection

December 2007; p ublished online November 2007 .

Journal Article. Subjects: Proteins. 4874 words.

Chemical conjugates of monoclonal antibodies with fluorophores or enzymes have long been used for diagnostic purposes and experimental therapeutic approaches. Recombinant technology allows...

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Absolute conservation of residue 6 of immunoglobulin heavy chain variable regions of class IIA is required for correct folding.

H J de Haard, B Kazemier, A van der Bent, P Oudshoorn, P Boender, B van Gemen, J W Arends and H R Hoogenboom.

in Protein Engineering, Design and Selection

December 1998; p ublished online December 1998 .

Journal Article. Subjects: Proteins. 0 words.

While studying the expression of single-chain antibodies (scFv) derived from several murine monoclonal antibodies, we found that residue 6 in Framework region 1 of the heavy chain variable...

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An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei l-lactate dehydrogenase activity induced by a single amino acid substitution

Kazuhito Arai, Atsushi Hishida, Mariko Ishiyama, Takeo Kamata, Hiroyuki Uchikoba, Shinya Fushinobu, Hiroshi Matsuzawa and Hayao Taguchi.

in Protein Engineering, Design and Selection

January 2002; p ublished online January 2002 .

Journal Article. Subjects: Proteins. 5234 words.

Lactobacillus casei allosteric L-lactate dehydrogenase (l-LDH) absolutely requires fructose 1,6-bisphosphate [Fru(1,6)P2] for its catalytic activity under neutral conditions,...

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Abstracts of the 8th Annual Meeting of the Protein Engineering Society of Japan

in Protein Engineering, Design and Selection

September 1996 .

Journal Article. Subjects: Proteins. 0 words.

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Accelerating phage-display library selection by reversible and site-specific biotinylation

Akiko Koide, John Wojcik, Ryan N. Gilbreth, Annett Reichel, Jacob Piehler and Shohei Koide.

in Protein Engineering, Design and Selection

November 2009; p ublished online September 2009 .

Journal Article. Subjects: Proteins. 4184 words.

Immobilization of a target molecule to a solid support is an indispensable step in phage display library sorting. Here we describe an immobilization method that addresses shortcomings of...

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Accurate ab initio prediction of NMR chemical shifts of nucleic acids and nucleic acids/protein complexes

Andrea Victora, Heiko M. Möller and Thomas E. Exner.

in Nucleic Acids Research

December 2014; p ublished online November 2014 .

Journal Article. Subjects: Molecular and Cell Biology; Proteins; Molecular Biology and Genetics. 7813 words.

NMR chemical shift predictions based on empirical methods are nowadays indispensable tools during resonance assignment and 3D structure calculation of proteins. However, owing to the very...

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Acetylcholinesterase engineering for detection of insecticide residues

Yvan Boublik, Pascale Saint-Aguet, Andrée Lougarre, Muriel Arnaud, François Villatte, Sandino Estrada-Mondaca and Didier Fournier.

in Protein Engineering, Design and Selection

January 2002; p ublished online January 2002 .

Journal Article. Subjects: Proteins. 3723 words.

To detect traces of insecticides in the environment using biosensors, we engineered Drosophila acetylcholinesterase (AChE) to increase its sensitivity and its rate of phosphorylation or...

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Acknowledgements

in Protein Engineering, Design and Selection

December 1996 .

Journal Article. Subjects: Proteins. 0 words.

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Activation of E350A mutant maltodextrin phosphorylase by exogenously added acetate

Peter Drueckes and Reinhard Schinzel.

in Protein Engineering, Design and Selection

August 1996 .

Journal Article. Subjects: Proteins. 0 words.

Site-directed mutagenesis of E350 to alanine in Escherichia coli maltodextrin phosphorylase reduced both enzyme activity (100-fold) and apparent binding of the oligosaccharide substrate...

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Activation of the low oxygen affinity-inducing potential of the Asn108(β)→Lys mutation of Hb-Presbyterian on intramolecular αα-fumaryl cross-bridging

Belur N. Manjula, Ashok Malavalli, Muthuchidambaram Prabhakaran, Joel M. Friedman and A.Seetharama Acharya.

in Protein Engineering, Design and Selection

May 2001; p ublished online May 2001 .

Journal Article. Subjects: Proteins. 5394 words.

The Asn108β→Lys mutation in hemoglobin (HbPresbyterian mutation) endows a low O2 affinity-inducing propensity to the protein. Introduction of a fumaryl cross-bridge between its two α99...

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The active site of carboxypeptidase Taq possesses the active-site motif His–Glu–X–X-His of zinc-dependent endopeptidases and aminopeptidases

Sang-Hyeon Lee, Hayao Taguchi, Etsuro Yoshimura, Etsuo Minagawa, Shuichi Kaminogawa, M Hiroshi and Takahisa Ohta.

in Protein Engineering, Design and Selection

June 1996 .

Journal Article. Subjects: Proteins. 0 words.

Carboxypeptidase (CPase) Taq possesses the His–Glu–X–X–His sequence, which is the consensus sequence in the active site of zinc-dependent endopeptidases and amino-peptidases, at positions...

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The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169

Anu Koivula, Tapani Reinikainen, Laura Ruohonen, Anne Valkeajärvi, Marc Claeyssens, Olle Teleman, Gerard J. Kleywegt, Michael Szardenings, Juha Rouvinen, T.Alwyn Jones and Tuula T. Teeri.

in Protein Engineering, Design and Selection

August 1996 .

Journal Article. Subjects: Proteins. 0 words.

Trichoderma reesei cellobiohydrolase II (CBHII) is an exoglucanase cleaving primarily cellobiose units from the non-reducing end of cellulose chains. The β-l,4 glycosidic bond...

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Active-site residues are critical for the folding and stability of methylamine dehydrogenase

Dapeng Sun, Limei H. Jones, F.Scott Mathews and Victor L. Davidson.

in Protein Engineering, Design and Selection

September 2001; p ublished online September 2001 .

Journal Article. Subjects: Proteins. 6180 words.

Site-directed mutagenesis was used to alter active-site residues of methylamine dehydrogenase (MADH) from Paracoccus denitrificans. Four residues of the β subunit of MADH which are in close...

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Activity enhancement of CotA laccase by hydrophilic engineering, histidine tag optimization and static culture

Lei Li, Tian Xie, Zhongchuan Liu, Hong Feng and Ganggang Wang.

in Protein Engineering, Design and Selection

January 2018; p ublished online December 2017 .

Journal Article. Subjects: Proteins. 2249 words.

Abstract

CotA protein from Bacillus subtilis is of laccase activity. The solubility of recombinant CotA is low, which hinders its application. In this study,...

Activity of linked HIV-1 proteinase dimers containing mutations in the active site region

Péter Bagossi, Yin-Shyun E. Cheng, Stephen Oroszlan and József Tözsér.

in Protein Engineering, Design and Selection

November 1996 .

Journal Article. Subjects: Proteins. 0 words.

Mutations were introduced into the active site triplet (Asp–Thr–Gly) of one or both subunits of a linked dimer of human immunodeficiency virus type 1 proteinase. Mutation of Thr to Ser in...

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Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures

Taro Kanzaki, Shuzo Ushioku, Ayumi Nakagawa, Toshihiko Oka, Kazunobu Takahashi, Takashi Nakamura, Kunihiro Kuwajima, Akihiko Yamagishi and Masafumi Yohda.

in Protein Engineering, Design and Selection

May 2010; p ublished online February 2010 .

Journal Article. Subjects: Proteins. 5965 words.

Group II chaperonins exist in archaea and the eukaryotic cytosol, and mediate protein folding in an ATP-dependent manner. We have been studying the reaction mechanism of group II...

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Adaptation of a thermophilic enzyme, 3-isopropylmalate dehydrogenase, to low temperatures

Toshiharu Suzuki, Masako Yasugi, Fumio Arisaka, Akihiko Yamagishi and Tairo Oshima.

in Protein Engineering, Design and Selection

February 2001; p ublished online February 2001 .

Journal Article. Subjects: Proteins. 4698 words.

Random mutagenesis coupled with screening of the active enzyme at a low temperature was applied to isolate cold-adapted mutants of a thermophilic enzyme. Four mutant enzymes with enhanced...

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