Journal Article

Optimized and Validated HPLC Analysis of St. John's Wort Extract and Final Products by Simultaneous Determination of Major Ingredients

Konstantina Zeliou, Nikos I. Kontaxis, Evangelia Margianni, Christos Petrou and Fotini N. Lamari

in Journal of Chromatographic Science

Volume 55, issue 8, pages 805-812
Published in print September 2017 | ISSN: 0021-9665
Published online May 2017 | e-ISSN: 1945-239X | DOI: https://dx.doi.org/10.1093/chromsci/bmx040
Optimized and Validated HPLC Analysis of St. John's Wort Extract and Final Products by Simultaneous Determination of Major Ingredients

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Abstract

Aim of this work was to develop a validated high performance liquid chromatography method for the analysis of extracts and final products of St. John's wort, according to international guidelines for bioanalytical method validation. Chromatographic separation was performed on a C18 column with a combination of gradient and isocratic steps; the mobile phase composed of ammonium acetate solution (pH 4.5; 10 mM), acetonitrile and methanol. Quantification and method validation was performed using extract spiked with external reference standards of chlorogenic acid,...

Journal Article.  5711 words.  Illustrated.

Subjects: Chemistry

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