Journal Article

Rapid flow-cytometric susceptibility testing of Candida species

Bernard Rudensky, Ellen Broidie, Amos M. Yinnon, Tanya Weitzman, Esther Paz, Nathan Keller and David Raveh

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 1, pages 106-109
Published in print January 2005 | ISSN: 0305-7453
Published online January 2005 | e-ISSN: 1460-2091 | DOI:
Rapid flow-cytometric susceptibility testing of Candida species

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  • Medical Oncology
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Objectives: To develop a rapid flow-cytometric antifungal susceptibility test and to compare results with the standard methods.

Methods: Reference and laboratory strains of Candida were tested for susceptibility to fluconazole and echinocandin by fluorescent flow cytometry using Acridine Orange as indicator of viability. Flow cytometry results were compared with MICs as determined by macrodilution and/or Etest.

Results: Seventy Candida strains were tested for susceptibility to fluconazole, and 74 strains for susceptibility to echinocandin. Minimal concentration of fluconazole causing 40% cell damage, as determined by flow cytometry, showed excellent association with MIC, as determined by other methods. The flow method, completed within 5 h, had excellent sensitivity and specificity to distinguish between sensitive, susceptible dose-dependent and resistant strains. The flow cytometry method for echinocandin was completed within 3 h, and minimal concentration causing 50% cell damage was associated with MIC as determined by macrodilution.

Conclusions: Antifungal susceptibility testing by FACS is a reliable, rapid method for determining susceptibility of Candida to fluconazole and echinocandin. The method allows same-day results, assisting in the selection of appropriate antifungal therapy.

Keywords: fungal; MIC; FACS; fluconazole; echinocandin

Journal Article.  2231 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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