Journal Article

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells

Anestis Tsakiridis, Elena Tzouanacou, Afifah Rahman, Douglas Colby, Richard Axton, Ian Chambers, Valerie Wilson, Lesley Forrester and Joshua M. Brickman

in Nucleic Acids Research

Volume 37, issue 19, pages e129-e129
Published in print October 2009 | ISSN: 0305-1048
Published online August 2009 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/gkp640

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Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These polyadenylation (poly A) trap vectors employ a splice donor to capture an endogenous gene's polyadenylation sequence and provide transcript stability. However, the spectrum of mutations generated by these vectors appears largely restricted to the last intron of target loci due to nonsense-mediated...

Journal Article.  9522 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

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