Journal Article

Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection

Mariya Sambir, Larisa B. Ivanova, Anton V. Bryksin, Henry P. Godfrey and Felipe C. Cabello

in FEMS Microbiology Letters

Volume 317, issue 2, pages 172-180
Published in print April 2011 |
Published online February 2011 | e-ISSN: 1574-6968 | DOI: https://dx.doi.org/10.1111/j.1574-6968.2011.02226.x

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Abstract

Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to exploring the physiologic function of uvrABbu and its possible role in survival in the host in the face of DNA-damaging agents, a partially deleted uvrA mutant was isolated by targeted inactivation. While growth of this mutant was markedly inhibited by UV irradiation, mitomycin C (MMC) and hydrogen peroxide at doses that lacked effect on wild-type B. burgdorferi, its response to pH 6.0–6.8 and reactive nitrogen intermediates was similar to that of the wild-type parental strain. The sensitivity of the inactivation mutant to UV irradiation, MMC and peroxide was complemented by an extrachromosomal copy of uvrABbu. We conclude that uvrABbu is functional in B. burgdorferi.

Keywords: Borrelia burgdorferi; Lyme disease; uvrA; DNA damage; nucleotide excision repair; UV radiation

Journal Article.  4522 words.  Illustrated.

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