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N-Glycosidase–carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation

Emily M. Kwan, Alisdair B. Boraston, Bradley W. McLean, Douglas G. Kilburn and R. Antony J. Warren.

in Protein Engineering, Design and Selection

October 2005; p ublished online September 2005 .

Journal Article. Subjects: Proteins. 3907 words.

A carbohydrate-binding module (CBM) was fused to the N-termini of mannosyl-glycoprotein endo-β-N-acetylglucosaminidase (EndoF1) and peptide N-glycosidase F (PNGaseF), two glycosidases from null...

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Design of the linkers which effectively separate domains of a bifunctional fusion protein

Ryoichi Arai, Hiroshi Ueda, Atsushi Kitayama, Noriho Kamiya and Teruyuki Nagamune.

in Protein Engineering, Design and Selection

August 2001; p ublished online August 2001 .

Journal Article. Subjects: Proteins. 2978 words.

With the aim of separating the domains of a bifunctional fusion protein, the ability of several lengths of helix-forming peptides to separate two weakly interacting β-can domains was...

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Fusion to a highly stable consensus albumin binding domain allows for tunable pharmacokinetics

Steven A. Jacobs, Alan C. Gibbs, Michelle Conk, Fang Yi, Diane Maguire, Colleen Kane and Karyn T. O'Neil.

in Protein Engineering, Design and Selection

October 2015; p ublished online August 2015 .

Journal Article. Subjects: Proteins. 7088 words.

A number of classes of proteins have been engineered for high stability using consensus sequence design methods. Here we describe the engineering of a novel albumin binding domain (ABD)...

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Tuning the serum persistence of human serum albumin domain III:diabody fusion proteins

Vania E. Kenanova, Tove Olafsen, Felix B. Salazar, Lawrence E. Williams, Scott Knowles and Anna M. Wu.

in Protein Engineering, Design and Selection

October 2010; p ublished online August 2010 .

Journal Article. Subjects: Proteins. 7019 words.

The long circulation persistence of human serum albumin (HSA) is enabled by its domain III (DIII) interaction with the neonatal Fc receptor (FcRn). A protein scaffold based on HSA DIII was...

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Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage.

S W Polyak, G Forsberg, B E Forbes, K A McNeil, S E Aplin and J C Wallace.

in Protein Engineering, Design and Selection

June 1997; p ublished online June 1997 .

Journal Article. Subjects: Proteins. 0 words.

To improve site-specific cleavage of a methionyl porcine growth hormone [[Met1]-pGH(1-46)-IGF-II] fusion protein by the enzyme H64A subtilisin, a series of flexible, unstructured spacer...

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Use of protein A gene fusions for the analysis of structure-function relationship of the transactivator protein C of bacteriophage Mu.

A De, B D Paul, V Ramesh and V Nagaraja.

in Protein Engineering, Design and Selection

August 1997; p ublished online August 1997 .

Journal Article. Subjects: Proteins. 0 words.

A sensitive dimerization assay for DNA binding proteins has been developed using gene fusion technology. For this purpose, we have engineered a gene fusion using protein A gene of...

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Effects of novel peptides derived from the acidic tail of synuclein (ATS) on the aggregation and stability of fusion proteins

Sang Myun Park, Keun Jae Ahn, Han Young Jung, Jeon Han Park and Jongsun Kim.

in Protein Engineering, Design and Selection

March 2004; p ublished online March 2004 .

Journal Article. Subjects: Proteins. 6378 words.

The acidic tail of α‐synuclein (ATSα) has been shown to protect the glutathione S‐transferase (GST)–ATSα fusion protein from environmental stresses, such as heat, pH and metal ions. In this...

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Fusion of the antiferritin antibody VL domain to barnase results in enhanced solubility and altered pH stability

Sergey P. Martsev, Yaroslav I. Tsybovsky, Oleg A. Stremovskiy, Sergey G. Odintsov, Taras G. Balandin, Paolo Arosio, Zinaida I. Kravchuk and Sergey M. Deyev.

in Protein Engineering, Design and Selection

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Proteins. 6753 words.

Chimeric immunotoxins that combine antigen recognition domains of antibodies and cytotoxic RNases have attracted much attention in recent years as potential targeted agents for cancer...

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Topology of eukaryotic multispanning transmembrane proteins: use of LacZ fusions for the localization of cytoplasmic domains in COS.M6 cells.

R Schülein, C Rutz and W Rosenthal.

in Protein Engineering, Design and Selection

June 1997; p ublished online June 1997 .

Journal Article. Subjects: Proteins. 0 words.

In Escherichia coli, the topology of inner membrane proteins can be studied conveniently with the alkaline phosphatase/beta-galactosidase (PhoA/LacZ) gene fusion system. PhoA is...

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A strategy for optimizing the monodispersity of fusion proteins: application to purification of recombinant HPV E6 oncoprotein

Yves Nominé, Tutik Ristriani, Cécile Laurent, Jean-François Lefèvre, Étienne Weiss and Gilles Travé.

in Protein Engineering, Design and Selection

April 2001; p ublished online April 2001 .

Journal Article. Subjects: Proteins. 6459 words.

Recombinant production of HPV oncoprotein E6 is notoriously difficult. The unfused sequence is produced in inclusion bodies. By contrast, fusions of E6 to the C-terminus of carrier proteins...

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Design and analysis of post‐fusion 6‐helix bundle of heptad repeat regions from Newcastle disease virus F protein

Jieqing Zhu, Pengyun Li, Tinghe Wu, Feng Gao, Yi Ding, Catherine W.‐H. Zhang, Zihe Rao, George F. Gao and Po Tien.

in Protein Engineering, Design and Selection

May 2003; p ublished online May 2003 .

Journal Article. Subjects: Proteins. 3622 words.

Fusion of paramyxovirus to the cell involves receptor binding of the HN glycoprotein and a number of conformational changes of F glycoprotein. The F protein is expressed as a homotrimer on...

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Effect of protein fusion on the transition temperature of an environmentally responsive elastin‐like polypeptide: a role for surface hydrophobicity?

K. Trabbic‐Carlson, D.E. Meyer, L. Liu, R. Piervincenzi, N. Nath, T. LaBean and A. Chilkoti.

in Protein Engineering, Design and Selection

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Proteins. 9070 words.

The limited throughput, scalability and high cost of protein purification by chromatography provide motivation for the development of non‐chromatographic protein purification technologies...

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Stable linker peptides for a cellulose-binding domain–lipase fusion protein expressed in Pichia pastoris

Malin Gustavsson, Janne Lehtiö, Stuart Denman, Tuula T. Teeri, Karl Hult and Mats Martinelle.

in Protein Engineering, Design and Selection

September 2001; p ublished online September 2001 .

Journal Article. Subjects: Proteins. 3981 words.

Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim...

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Display of β-lactamase on the Escherichia coli surface: outer membrane phenotypes conferred by Lpp′–OmpA′–β-lactamase fusions

George Georgiou, Daren L. Stephens, Christos Stathopoulos, Heather L. Poetschke, John Mendenhall and Charles F. Earhart.

in Protein Engineering, Design and Selection

February 1996 .

Journal Article. Subjects: Proteins. 0 words.

Bacterial cell-surface exposure of foreign peptides and soluble proteins has been achieved recently by employing a fusion protein methodology. An Lpp′–OmpA(46–159)–Bla fusion protein has...

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The IgM CH2 domain as covalently linked homodimerization module for the generation of fusion proteins with dual specificity

Oliver Seifert, Aline Plappert, Nadine Heidel, Sina Fellermeier, Sylvia K.E. Messerschmidt, Fabian Richter and Roland E. Kontermann.

in Protein Engineering, Design and Selection

October 2012; p ublished online September 2012 .

Journal Article. Subjects: Proteins. 4720 words.

Dimeric assembly of antibody fragments and other therapeutic molecules can result in increased binding and improved bioactivity. Here, we investigated the use of the IgM heavy chain domain...

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Immunoglobulin Fc domain fusion to apolipoprotein(a) kringle V significantly prolongs plasma half-life without affecting its anti-angiogenic activity

Hyun-Kyung Yu, Ho-Jeong Lee, Jin-Hyung Ahn, In-Hwan Lim, Jae-Hoon Moon, Yeup Yoon, Lee S.H. Yi, Sun Jin Kim and Jang-Seong Kim.

in Protein Engineering, Design and Selection

June 2013; p ublished online April 2013 .

Journal Article. Subjects: Proteins. 5306 words.

Angiogenesis is crucial for tumor growth and metastasis. Blocking this process is, therefore, a potentially powerful approach for the treatment of cancer. Human apolipoprotein(a) kringle V...

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Fusion of a recombinant antibody fragment with a homo-amino-acid polymer: effects on biophysical properties and prolonged plasma half-life

Martin Schlapschy, Ina Theobald, Hildegard Mack, Margret Schottelius, Hans-Jürgen Wester and Arne Skerra.

in Protein Engineering, Design and Selection

June 2007; p ublished online June 2007 .

Journal Article. Subjects: Proteins. 10307 words.

Chemical conjugation of small recombinant proteins with polyethylene glycol (PEG) is an established strategy to extend their typically short circulation times to a therapeutically useful...

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Half-life extension of a single-chain diabody by fusion to domain B of staphylococcal protein A

Felix Unverdorben, Aline Färber-Schwarz, Fabian Richter, Meike Hutt and Roland E. Kontermann.

in Protein Engineering, Design and Selection

February 2012; p ublished online January 2012 .

Journal Article. Subjects: Proteins. 4507 words.

Binding of a therapeutic protein to a long-circulating plasma protein can result in a strongly extended half-life. Among these plasma proteins, albumin and immunoglobulins are of special...

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Functional phage display of two murine α/β T-cell receptors is strongly dependent on fusion format, mode and periplasmic folding assistance

G.Å. Løset, E. Lunde, B. Bogen, O.H. Brekke and I. Sandlie.

in Protein Engineering, Design and Selection

September 2007; p ublished online October 2007 .

Journal Article. Subjects: Proteins. 8539 words.

Phage display has been instrumental for the success of antibody (Ab) technology. The aim of the present study was to explore phage display of soluble T-cell receptors (TCRs). A library...

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Efficient site specific removal of a C-terminal FLAG fusion from a Fab′ using copper(II) ion catalysed protein cleavage

David P. Humphreys, Bryan J. Smith, Lloyd M. King, Shauna M. West, Dominic G. Reeks and Paul E. Stephens.

in Protein Engineering, Design and Selection

February 1999; p ublished online February 1999 .

Journal Article. Subjects: Proteins. 5174 words.

The peptide sequence NDKTHC was investigated as a site for efficient, specific cleavage of a fusion protein by cupric ions using a humanised γ1 Fab′ as a model protein. The native upper...

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