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The effects of helix breaking mutations in the diphtheria toxin transmembrane domain helix layers of the fusion toxin DAB389IL-2.

H Y Hu, P D Huynh, J R Murphy and J C vanderSpek.

in Protein Engineering, Design and Selection

January 1998; p ublished online January 1998 .

Journal Article. Subjects: Proteins. 0 words.

The fusion protein toxin DAB389IL-2 is composed of the catalytic and transmembrane domains of diphtheria toxin genetically linked to human interleukin 2 (IL-2). This fusion toxin is...

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A33scFv–Green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting

Ulf Petrausch, Jens Dernedde, Vânia Coelho, Hossein Panjideh, Dietmar Frey, Hendrik Fuchs, Eckhard Thiel and P. Markus Deckert.

in Protein Engineering, Design and Selection

December 2007; p ublished online November 2007 .

Journal Article. Subjects: Proteins. 4874 words.

Chemical conjugates of monoclonal antibodies with fluorophores or enzymes have long been used for diagnostic purposes and experimental therapeutic approaches. Recombinant technology allows...

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Anti-glycophorin single-chain Fv fusion to low-affinity mutant erythropoietin improves red blood cell-lineage specificity

Noah D. Taylor, Jeffrey C. Way, Pamela A. Silver and Pablo Cironi.

in Protein Engineering, Design and Selection

April 2010; p ublished online January 2010 .

Journal Article. Subjects: Proteins. 6258 words.

The presence of erythropoietin (Epo) receptors on cells besides red blood cell precursors, such as cancer cells or megakaryocyte precursors, can lead to side effects during Epo therapy...

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Anti-angiogenic effect of an insertional fusion protein of human basic fibroblast growth factor and ribonuclease-1

Tetsu Hayashida, Masakazu Ueda, Koichi Aiura, Hiroko Tada, Masayuki Onizuka, Masaharu Seno, Hidenori Yamada and Masaki Kitajima.

in Protein Engineering, Design and Selection

July 2005; p ublished online June 2005 .

Journal Article. Subjects: Proteins. 4939 words.

Human pancreatic ribonuclease-1 (RNase1) does not exhibit its cytotoxicity unless it is artificially internalized into the cytosol. Furthermore, once it encounters the cytosolic RNase...

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Different tissue distribution properties for glycosylation variants of fusion proteins containing the p40 subunit of murine interleukin-12

F. Bootz, D. Venetz, B. Ziffels and D. Neri.

in Protein Engineering, Design and Selection

October 2016; p ublished online September 2016 .

Journal Article. Subjects: Proteins. 5087 words.

Antibody-based fusion proteins are gaining increasing importance for therapeutic applications, but the impact of glycosylation on in vivo biopharmaceutical performance is not always...

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Targeting glioblastoma multiforme with an IL-13/diphtheria toxin fusion protein in vitro and in vivo in nude mice

Chunbin Li, Walter A. Hall, Ni Jin, Deborah A. Todhunter, Angela Panoskaltsis-Mortari and Daniel A. Vallera.

in Protein Engineering, Design and Selection

May 2002; p ublished online May 2002 .

Journal Article. Subjects: Proteins. 6092 words.

Fusion proteins composed of tumor binding agents and potent catalytic toxins show promise for intracranial therapy of brain cancer and an advantage over systemic therapy. Glioblastoma...

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Construction and optimization of a CC49-Based scFv-β-lactamase fusion protein for ADEPT

Martin Roberge, Melodie Estabrook, Joshua Basler, Regina Chin, Pete Gualfetti, Amy Liu, Stephanie B. Wong, M. Harunur Rashid, Tom Graycar, Lilia Babé and Volker Schellenberger.

in Protein Engineering, Design and Selection

April 2006; p ublished online January 2006 .

Journal Article. Subjects: Proteins. 3700 words.

CC49 is a clinically validated antibody with specificity for TAG-72, a carbohydrate epitope that is over-expressed and exposed on a large fraction of solid malignancies. We constructed a...

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Solubilization of aggregation-prone heterologous proteins by covalent fusion of stress-responsive Escherichia coli protein, SlyD

Kyung-Yeon Han, Jong-Am Song, Keum-Young Ahn, Jin-Seung Park, Hyuk-Seong Seo and Jeewon Lee.

in Protein Engineering, Design and Selection

November 2007; p ublished online October 2007 .

Journal Article. Subjects: Proteins. 4029 words.

The proteome profile of Escherichia coli BL21(DE3) generated in response to heat shock stress was analyzed by two-dimensional electrophoresis (2-DE), wherein we identified a FKBP-type...

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Efficient production of the C-terminal domain of secretory leukoprotease inhibitor as a thrombin-cleavable fusion protein in Escherichia coli

Ken-ichi Masuda, Takashi Kamimura, Masaharu Kanesaki, Koji Ishii, Atsushi Imaizumi, Takashi Sugiyama, Yoji Suzuki and Eiko Ohtsuka.

in Protein Engineering, Design and Selection

January 1996 .

Journal Article. Subjects: Proteins. 0 words.

We have developed a high-level production system for the C-terminal domain of secretory leukoprotease inhibitor (SLPI) to investigate its pharmacological activities. A gene for the...

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Selection of cadmium specific hexapeptides and their expression as OmpA fusion proteins in Escherichia coli.

M Mejàre, S Ljung and L Bülow.

in Protein Engineering, Design and Selection

June 1998; p ublished online June 1998 .

Journal Article. Subjects: Proteins. 0 words.

In searching for novel peptides with affinity for cadmium, the phage display technique was utilized. In the selection procedure, cadmium ions were immobilized on a metal chelating Sepharose...

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A strategy for high-level expression of soluble and functional human interferon α as a GST-fusion protein in E.coli

Imen Rabhi-Essafi, Amine Sadok, Noureddine Khalaf and Dahmani M. Fathallah.

in Protein Engineering, Design and Selection

May 2007; p ublished online April 2007 .

Journal Article. Subjects: Proteins. 5916 words.

Escherichia coli is the most extensively used host for the production of recombinant proteins. However, most of the eukaryotic proteins are typically obtained as insoluble,...

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Targeting myeloid leukemia with a DT390-mIL-3 fusion immunotoxin: ex vivo and in vivo studies in mice

Daniel A. Vallera, Su-Yeong Seo, Angela Panoskaltsis-Mortari, James D. Griffin and Bruce R. Blazar.

in Protein Engineering, Design and Selection

September 1999; p ublished online September 1999 .

Journal Article. Subjects: Proteins. 5368 words.

The IL-3 receptor was expressed on a high frequency of myeloid leukemia cells and also on hematopoietic and vascular cells. We previously showed that a recombinant IL-3 fusion immunotoxin...

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Affinity enhancement of a recombinant antibody: formation of complexes with multiple valency by a single-chain Fv fragment–core streptavidin fusion

Sergey M. Kipriyanov, Melvyn Little, Harald Kropshofer, Frank Breitling, Stefanie Gotter and Stefan Dübel.

in Protein Engineering, Design and Selection

February 1996 .

Journal Article. Subjects: Proteins. 0 words.

In antigen–antibody interactions, the high avidity of antibodies depends on the affinity and number of the individual binding sites. To develop artificial antibodies with multiple valency,...

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Human pancreatic RNase1-human epidermal growth factor fusion: an entirely human 'immunotoxin analog' with cytotoxic properties against squamous cell carcinomas.

K Psarras, M Ueda, T Yamamura, S Ozawa, M Kitajima, S Aiso, S Komatsu and M Seno.

in Protein Engineering, Design and Selection

December 1998; p ublished online December 1998 .

Journal Article. Subjects: Proteins. 0 words.

The gene encoding human pancreatic ribonuclease 1 (hpRNasel) was fused with a gene encoding human epidermal growth factor (hEGF). The hybrid human protein was isolated from Escherichia coli...

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Hydrophobicity engineering of cholera toxin A1 subunit in the strong adjuvant fusion protein CTA1-DD

Lena Ågren, Martin Norin, Nils Lycke and Björn Löwenadler.

in Protein Engineering, Design and Selection

February 1999; p ublished online February 1999 .

Journal Article. Subjects: Proteins. 4635 words.

Protein engineering of the cholera toxin A1 subunit (CTA1) fused to a dimer of the Ig-binding D-region of Staphylococcus aureus protein A (DD) was employed to investigate the effect of...

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Generation of a dual-functional split-reporter protein for monitoring membrane fusion using self-associating split GFP

Hirohito Ishikawa, Fanxia Meng, Naoyuki Kondo, Aikichi Iwamoto and Zene Matsuda.

in Protein Engineering, Design and Selection

December 2012; p ublished online August 2012 .

Journal Article. Subjects: Proteins. 5237 words.

Split reporter proteins capable of self-association and reactivation have applications in biomedical research, but designing these proteins, especially the selection of appropriate split...

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Genetically engineered brain drug delivery vectors: cloning, expression and in vivo application of an anti-transferrin receptor single chain antibody–streptavidin fusion gene and protein

Jian Yi Li, Keijiro Sugimura, Ruben J.Boado, Hwa Jeong Lee, Crystal Zhang, Stefan Duebel and William M.Pardridge.

in Protein Engineering, Design and Selection

September 1999; p ublished online September 1999 .

Journal Article. Subjects: Proteins. 8114 words.

A single chain Fv antibody–streptavidin fusion protein was expressed and purified from bacterial inclusion bodies following cloning of the genes encoding the variable region of the heavy...

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SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies

Jonathan H. Davis, Christel Aperlo, Yue Li, Emmi Kurosawa, Yan Lan, Kin-Ming Lo and James S. Huston.

in Protein Engineering, Design and Selection

April 2010; p ublished online February 2010 .

Journal Article. Subjects: Proteins. 5219 words.

Bispecific antibodies and asymmetric Fc fusion proteins offer opportunities for important advances in therapeutics. Bivalent IgG depends upon in vivo dimerization of its heavy chains,...

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An evaluation tool for FKBP12-dependent and -independent mTOR inhibitors using a combination of FKBP-mTOR fusion protein, DSC and NMR

Mitsuhiro Sekiguchi, Yoshihiro Kobashigawa, Masashi Kawasaki, Masashi Yokochi, Tetsuo Kiso, Ken-ichi Suzumura, Keitaro Mori, Toshio Teramura and Fuyuhiko Inagaki.

in Protein Engineering, Design and Selection

November 2011; p ublished online September 2011 .

Journal Article. Subjects: Proteins. 3369 words.

Mammalian target of rapamycin (mTOR), a large multidomain protein kinase, regulates cell growth and metabolism in response to environmental signals. The FKBP rapamycin-binding (FRB) domain...

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Generation of AcGFP fusion with single-chain Fv selected from a phage display library constructed from mice hyperimmunized against 5-methyl 2′-deoxycytidine

Motohiro Ohshima, Kazuyuki Inoue, Hideki Hayashi, Daiki Tsuji, Michinao Mizugaki and Kunihiko Itoh.

in Protein Engineering, Design and Selection

November 2010; p ublished online September 2010 .

Journal Article. Subjects: Proteins. 5004 words.

DNA methylation is involved in many diseases such as cancer and autoimmunity. We generated recombinant single-chain Fv (scFv) antibodies against 5-methyl-2′-deoxycytidine (m5dCyd) using...

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