Experimental single-strain mobilomics reveals events that shape pathogen emergence
in Nucleic Acids Research
August 2016; p ublished online July 2016 .
Journal Article. Subjects: Genetics and Genomics; Research Methods in Life Sciences. 7055 words.
Virulence genes on mobile DNAs such as genomic islands (GIs) and plasmids promote bacterial pathogen emergence. Excision is an early step in GI mobilization, producing a circular GI and a...
Go to » full text: open access
Mg2+-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence
in Nucleic Acids Research
January 2015; p ublished online December 2014 .
Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 8980 words.
Among the deoxyribozymes catalyzing the ligation of two RNA substrates, 7S11 generates a branched RNA containing a 2′,5′-linkage. We have attached the small fluorogenic probe Bimane to the...
Go to » full text: open access
Tri-nucleotide threading for parallel amplification of minute amounts of genomic DNA
in Nucleic Acids Research
January 2006; p ublished online January 2006 .
Journal Article. Subjects: Genetics and Genomics; Research Methods in Life Sciences. 4905 words.
Efforts to correlate genetic variations with phenotypic differences are intensifying due to the availability of high-density maps of single nucleotide polymorphisms (SNPs) and the...
Go to » full text: open access
Enhanced detection of microsatellite instability using pre-PCR elimination of wild-type DNA homo-polymers in tissue and liquid biopsies
in Nucleic Acids Research
July 2018; p ublished online April 2018 .
Journal Article. Subjects: Genetics and Genomics; Research Methods in Life Sciences. 4753 words.
Abstract
Detection of microsatellite-instability in colonoscopy-obtained polyps, as well as in plasma-circulating DNA, is frequently confounded by sensitivity...
Identification of Noninvasive Biomarkers for Nephrotoxicity Using HK-2 Human Kidney Epithelial Cells
in Toxicological Sciences
August 2014; p ublished online August 2014 .
Journal Article. Subjects: Medical Toxicology; Research Methods in Life Sciences. 6112 words.
The kidney is an important site of xenobiotic-induced toxicity. Because the traditional markers of renal injury indicate only severe renal damage, new biomarkers are needed for a more...
Four-dimensional magnetic resonance imaging-derived ascending aortic flow eccentricity and flow compression are linked to aneurysm morphology
in Interactive CardioVascular and Thoracic Surgery
May 2015; p ublished online January 2015 .
Journal Article. Subjects: Cardiovascular Medicine; Research Methods in Life Sciences. 4106 words.
OBJECTIVES
The impact of specific blood flow patterns within ascending aortic and/or aortic root aneurysms on aortic morphology is unknown. We investigated...
3′LIFE: a functional assay to detect miRNA targets in high-throughput
in Nucleic Acids Research
September 2014; p ublished online July 2014 .
Journal Article. Subjects: Research Methods in Life Sciences; Genetics and Genomics. 12640 words.
MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene output at the post-transcriptional level by targeting degenerate elements primarily in 3′untranslated regions (3′UTRs) of...
Go to » full text: open access
Engineering a high-affinity methyl-CpG-binding protein
in Nucleic Acids Research
August 2006; p ublished online August 2006 .
Journal Article. Subjects: Research Methods in Life Sciences; Proteins; Molecular Biology and Genetics. 4350 words.
Core members of the MBD protein family (MeCP2, MBD1, MBD2 and MBD4) share a methyl-CpG-binding domain that has a specific affinity for methylated CpG sites in double-stranded DNA. By...
Go to » full text: open access
Real-time investigation of nucleic acids phosphorylation process using molecular beacons
in Nucleic Acids Research
June 2005; p ublished online June 2005 .
Journal Article. Subjects: Research Methods in Life Sciences; Enzymology; Molecular Biology and Genetics. 4199 words.
Phosphorylation of nucleic acids is an indispensable process to repair strand interruption of nucleic acids. We have studied the process of phosphorylation using molecular beacon (MB) DNA...
Go to » full text: open access
A novel high-throughput activity assay for the Trypanosoma brucei editosome enzyme REL1 and other RNA ligases
in Nucleic Acids Research
February 2016; p ublished online September 2015 .
Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Research Methods in Life Sciences. 7890 words.
The protist parasite Trypanosoma brucei causes Human African trypanosomiasis (HAT), which threatens millions of people in sub-Saharan Africa. Without treatment the infection is almost...
Go to » full text: open access
SL-quant: a fast and flexible pipeline to quantify spliced leader trans-splicing events from RNA-seq data
in GigaScience
P ublished online July 2018 .
Journal Article. Subjects: Biological Sciences; Bioinformatics and Computational Biology; Evolutionary Biology; Genetics and Genomics; Research Methods in Life Sciences. 3843 words.
Abstract
Background
The spliceosomal transfer of a short spliced leader (SL) RNA to an independent pre-mRNA...
Go to » full text: open access
The validity of the original EuroSCORE and EuroSCORE II in patients over the age of seventy
in Interactive CardioVascular and Thoracic Surgery
February 2015; p ublished online October 2014 .
Journal Article. Subjects: Molecular Biology and Genetics; Molecular and Cell Biology; Cardiovascular Medicine; Research Methods in Life Sciences. 3077 words.
OBJECTIVES
EuroSCORE II, despite improving on the original EuroSCORE system, has not solved all the calibration and predictability issues. We investigated...
