DNA fingerprint
Overview page. Subjects: Ecology and Conservation.
A set of DNA-based characters which are in a combination unique, or nearly so, to an individual organism. See also satellite DNA.
TE-AFLP: combining rapidity and robustness in DNA fingerprinting
in Nucleic Acids Research
December 2000; p ublished online December 2000 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 2988 words.
A new type of fingerprinting technique is presented, based on amplified fragment length polymorphism (AFLP). Rather than two endonucleases as in AFLP, we propose the use of three enzymes,...
A PCR-Based DNA Fingerprinting Technique: AFLP for Molecular Typing of Bacteria
in Nucleic Acids Research
September 1996; p ublished online September 1996 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 860 words.
Amplified restriction fragment polymorphism (AFLP) is a PCR-based DNA fingerprinting technique. In AFLP analysis, bacterial genomic DNA is digested with restriction enzymes, ligated to...
cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes
in Nucleic Acids Research
February 1999; p ublished online February 1999 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 3079 words.
A cDNA fingerprinting strategy was developed to identify genes based on their differential expression pattern during osteoblast development. Preliminary biological and molecular staging of...
Quantitative target display: a method to screen yeast mutants conferring quantitative phenotypes by ‘mutant DNA fingerprints’
in Nucleic Acids Research
September 2001; p ublished online September 2001 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 3719 words.
Whole genome sequencing of several microbes has revealed thousands of genes of unknown function. A large proportion of these genes seem to confer subtle quantitative phenotypes or phenotypes...
Screening of Differentially Amplified cDNA Products from RNA Arbitrarily Primed PCR Fingerprints Using Single Strand Conformation Polymorphism (SSCP) Gels
in Nucleic Acids Research
April 1996; p ublished online April 1996 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 3184 words.
Arbitrarily primed PCR fingerprinting of RNA and differential display resolved on an acrylamide gel has been extensively used to detect differentially expressed RNAs. However, after a...
In-gel DNA radiolabelling and two-dimensional pulsed field gel electrophoresis procedures suitable for fingerprinting and mapping small eukaryotic genomes
in Nucleic Acids Research
May 2000; p ublished online May 2000 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 2811 words.
A simple method for complete genome radiolabelling is described, involving long-wave UV exposure of agarose-embedded chromosomal DNA and [α-32P]dCTP incorporation mediated by the Klenow...
Minisatellite and HPRT Mutations in V79 and Human Cells Irradiated with Gamma Rays
in Radiation Protection Dosimetry
June 2002; p ublished online June 2002 .
Journal Article. Subjects: Nuclear Chemistry, Photochemistry, and Radiation. 0 words.
The induction of mutations at the Hprt locus and minisatellite sequences was studied in V79 cells, peripheral blood lymphocytes (PBL) and lymphoblastoid cells (CCRF-CEM) exposed to gamma...
Go to » abstract
Rapid determination of the complexity of cDNA bands extracted from DDRT—PCR polyacrylamide gels
in Nucleic Acids Research
September 1997; p ublished online September 1997 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 1993 words.
A band extracted from a differential display polyacrylamide gel often represents a composite of heterogeneous products. We have developed a non-radioactive method to simply and rapidly...
A high-throughput next-generation sequencing-based method for detecting the mutational fingerprint of carcinogens
in Nucleic Acids Research
August 2012; p ublished online June 2012 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 7271 words.
Many carcinogens leave a unique mutational fingerprint in the human genome. These mutational fingerprints manifest as specific types of mutations often clustering at certain genomic loci in...
Go to » full text: open access
Methylome profiling of cancer cells by amplification of inter-methylated sites (AIMS)
in Nucleic Acids Research
April 2002; p ublished online April 2002 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 4932 words.
Alterations of the DNA methylation pattern have been related to generalized chromosomal disruption and inactivation of multiple tumor suppressor genes in neoplasia. To screen for...
A new and versatile method for the successful conversion of AFLP™ markers into simple single locus markers
in Nucleic Acids Research
May 2003; p ublished online May 2003 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 5666 words.
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the...
Comparative gene expression profiling by oligonucleotide fingerprinting
in Nucleic Acids Research
May 1998; p ublished online May 1998 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 3936 words.
The use of hybridisation of synthetic oligonucleotides to cDNAs under high stringency to characterise gene sequences has been demonstrated by a number of groups. We have used two cDNA...
Classified fingerprinting: A method of comprehensive analysis for comparing megabase genomes
in Nucleic Acids Symposium Series
November 2001; p ublished online November 2001 .
Journal Article. Subjects: Chemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 0 words.
The objective of the work we describe is to establish elementary methods for investigating the functions of genes; specifically, a fingerprinting method for analyzing entire DNA fragments...
Go to » abstract
Non-stoichiometric reduced complexity probes for cDNA arrays
in Nucleic Acids Research
September 1998; p ublished online September 1998 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 6571 words.
A method is presented in which the reduced complexity and non-stoichiometric amplification intrinsic to RNA arbitrarily primed PCR fingerprinting (RAP-PCR) is used to advantage to generate...
Genomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long‐term (decades) desiccation but is protected from oxidative damage and degradation
in Nucleic Acids Research
June 2003; p ublished online June 2003 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 7736 words.
Genomic DNA of Nostoc commune (Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N‐p...
DNA sequencing by MALDI-TOF MS using alkali cleavage of RNA/DNA chimeras
in Nucleic Acids Research
April 2007; p ublished online April 2007 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 3585 words.
Approaches developed for sequencing DNA with detection by mass spectrometry use strategies that deviate from the Sanger-type methods. Procedures demonstrated so far used the sequence...
TRDB—The Tandem Repeats Database
in Nucleic Acids Research
P ublished online December 2006 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 4914 words.
Tandem repeats in DNA have been under intensive study for many years, first, as a consequence of their usefulness as genomic markers and DNA fingerprints and more recently as their role in...
Direct identification of differentially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers
in Nucleic Acids Research
June 1997; p ublished online June 1997 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 1450 words.
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially expressed genes by comparative display of arbitrarily amplified cDNA subsets. This...
Nucleic Acid Scanning-by-Hybridization of Enterohemorrhagic Escherichia Coli Isolates Using Oligodeoxynucleotide Arrays
in Nucleic Acids Research
December 1996; p ublished online December 1996 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 1417 words.
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid...
Targeted detection of in vivo endogenous DNA base damage reveals preferential base excision repair in the transcribed strand
in Nucleic Acids Research
January 2012; p ublished online September 2011 .
Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 9055 words.
Endogenous DNA damage is removed mainly via base excision repair (BER), however, whether there is preferential strand repair of endogenous DNA damage is still under intense debate. We...
Go to » full text: open access
