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fusion

Overview page. Subjects: Medicine and Health.

n. the joining together of two structures. For example, the surgical fusion of two or more vertebrae is performed to stabilize an unstable spine. Fusion of the epiphyses during...

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In situ imaging and isolation of proteins using dsDNA oligonucleotides

Graham Dellaire, Rozalia Nisman, Christopher H. Eskiw and David P. Bazett-Jones.

in Nucleic Acids Research

November 2004; p ublished online November 2004 .

Journal Article. Subjects: Proteins; Molecular Biology and Genetics. 7489 words.

As proteomics initiatives mature, the need will arise for the multiple visualization of proteins and supramolecular complexes within their true context, in situ. Single-stranded DNA and RNA...

A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

Yan Wang, Dennis E. Prosen, Li Mei, John C. Sullivan, Michael Finney and Peter B. Vander Horn.

in Nucleic Acids Research

February 2004; p ublished online February 2004 .

Journal Article. Subjects: Proteins; Molecular Biology and Genetics. 8384 words.

Mechanisms that allow replicative DNA polymerases to attain high processivity are often specific to a given polymerase and cannot be generalized to others. Here we report a protein...

A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda

Christine N. Zanghi, Heather A. Lankes, Birgit Bradel-Tretheway, Jessica Wegman and Stephen Dewhurst.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Proteins; Molecular Biology and Genetics. 5041 words.

Bacteriophage lambda (λ) permits the display of many foreign peptides and proteins on the gpD major coat protein. However, some recombinant derivatives of gpD are incompatible with the...

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Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP→YFP→mRFP FRET detected by flow cytometry

Liusheng He, Xiaoli Wu, James Simone, Derek Hewgill and Peter E. Lipsky.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Proteins; Molecular Biology and Genetics. 6514 words.

The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between...

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