January 2013; p ublished online September 2012 .
Journal Article. Subjects: Carbohydrates. 9247 words.
Toxoplasma gondii is the causative agent of toxoplasmosis, one of the most widespread infections in humans and animals, and is a major opportunistic pathogen in...
Proteoglycans from Boswellia serrata Roxb. and B. carteri Birdw. and identification of a proteolytic plant basic secretory protein
November 2012; p ublished online July 2012 .
Journal Article. Subjects: Carbohydrates. 8688 words.
Water-soluble high molecular weight compounds were isolated in yields of 21–22% from the oleogum of Boswellia serrata and B. carteri. Using anion exchange chromatography and gel permeation...
August 2004; p ublished online April 2004 .
Journal Article. Subjects: Carbohydrates. 3515 words.
Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which...
August 2009; p ublished online May 2009 .
Journal Article. Subjects: Carbohydrates. 10288 words.
Protein O-mannosylation is an essential modification in fungi and animals. Different from most other types of O-glycosylation, protein O-mannosylation is initiated in the endoplasmic...
November 2005; p ublished online June 2005 .
Journal Article. Subjects: Carbohydrates. 8253 words.
The human mucin MUC1 is expressed both as a transmembrane heterodimeric protein complex that recycles via the trans-Golgi network (TGN) and as a secreted isoform. To determine whether...
September 2016; p ublished online October 2016 .
Journal Article. Subjects: Carbohydrates. 12565 words.
Protein glycosylation is an essential co- and post-translational modification of secretory and membrane proteins in all eukaryotes. The initial steps of N-glycosylation and N-glycan...
N-Glycan synthesis in the apical and basolateral secretory pathway of epithelial MDCK cells and the influence of a glycosaminoglycan domain
November 2011; p ublished online July 2011 .
Journal Article. Subjects: Carbohydrates. 6670 words.
Different classes of glycans are implicated as mediators of apical protein sorting in the secretory pathway of epithelial cells, but recent research indicates that sorting to the apical and...
September 2007; p ublished online June 2007 .
Journal Article. Subjects: Carbohydrates. 5811 words.
VIP36 is an intracellular lectin that cycles between the endoplasmic reticulum (ER) and the Golgi apparatus, and is thought to act as a cargo receptor in the transport and sorting of...
Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins
February 2002; p ublished online February 2002 .
Journal Article. Subjects: Carbohydrates. 9349 words.
Recently, complex O-glycosylation of the cytoplasmic/nuclear protein Skp1 has been characterized in the eukaryotic microorganism Dictyostelium. Skp1’s glycosylation is mediated by the...
A Tetraantennary Glycan with Bisecting N-Acetylglucosamine and the Sda Antigen is the Predominant N-Glycan on Bovine Pregnancy-Associated Glycoproteins
January 2008; p ublished online October 2007 .
Journal Article. Subjects: Carbohydrates. 6457 words.
Pregnancy-associated glycoproteins (PAGs) are major secretory proteins of trophoblast cells in ruminants. Binucleate trophoblast giant cells (BNCs) store these proteins in secretory...
The Alg5 ortholog Wollknäuel is essential for correct epidermal differentiation during Drosophila late embryogenesis
June 2011; p ublished online January 2011 .
Journal Article. Subjects: Carbohydrates. 8915 words.
The formation of an extracellular matrix (ECM) presupposes an ordered delivery of its components to ensure its stereotypic architecture. The Drosophila cuticle is an ECM produced by the...
Dolichol phosphate mannose synthase from the filamentous fungus Trichoderma reesei belongs to the human and Schizosaccharomyces pombe class of the enzyme
October 2000; p ublished online October 2000 .
Journal Article. Subjects: Carbohydrates. 5757 words.
Dolichol phosphate mannose (DPM) synthase activity, which is required in N-glycosylation, O-mannosylation, and glycosylphosphatidylinositol membrane anchoring of protein, has been postulated...
July 2013; p ublished online April 2013 .
Journal Article. Subjects: Carbohydrates. 6851 words.
The leguminous-type (L-type) lectin ER-Golgi intermediate compartment (ERGIC)-53, a homo-oligomeric endoplasmic reticulum (ER)-Golgi recycling protein, functions as a transport receptor for...
September 2016; p ublished online October 2016 .
Journal Article. Subjects: Carbohydrates. 9428 words.
Plant endomembranes are required for the biosynthesis and secretion of complex cell wall matrix polysaccharides, glycoproteins and proteoglycans. To define the biochemical roadmap that...
Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris
September 1998; p ublished online September 1998 .
Journal Article. Subjects: Carbohydrates. 4981 words.
A stable GS115 Pichia pastoris recombinant strain was constructed to secrete a truncated form of the human α(1,3/4) fucosyltransferase (amino acids 45–361). Enzyme production resulted from...
June 1996 .
Journal Article. Subjects: Carbohydrates. 0 words.
The Saccharomyces cerevisiae alg3-1 mutant is descilbed as defective in the biosynthesis of dolichol-linked oligosaccharides (Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80,...
June 2005; p ublished online January 2005 .
Journal Article. Subjects: Carbohydrates. 6517 words.
Rat endomannosidase is a glycosidic enzyme that catalyzes the cleavage of di-, tri-, or tetrasaccharides (Glc1–3Man), from N-glycosylation intermediates with terminal glucose residues. To...
The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation
March 1999; p ublished online March 1999 .
Journal Article. Subjects: Carbohydrates. 7567 words.
The Saccharomyces cerevisiae mutant cwh8 was previously found to have an anomalous cell wall. Here we show that the cwh8 mutant has an N-glycosylation defect. We found that cwh8 cells were...
Reduced expression of the O-mannosyltransferase 2 (AfPmt2) leads to deficient cell wall and abnormal polarity in Aspergillus fumigatus
May 2010; p ublished online January 2010 .
Journal Article. Subjects: Carbohydrates. 7165 words.
Protein O-mannosyltransferases (PMTs) initiate O-mannosylation of secretory proteins, which are of fundamental importance in eukaryotes. The human fungal pathogen Aspergillus fumigatus poss...
Protein core-dependent glycosaminoglycan modification and glycosaminoglycan-dependent polarized sorting in epithelial Madin–Darby canine kidney cells
April 2011; p ublished online November 2010 .
Journal Article. Subjects: Carbohydrates. 6468 words.
The proteoglycan serglycin (SG) fused to green fluorescent protein (GFP) is secreted predominantly from the apical surface of polarized epithelial Madin–Darby canine kidney (MDCK) cell...