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sensitive

Overview page. Subjects: Medicine and Health.

adj. possessing the ability to respond to a stimulus. The cells of the retina, for example, are sensitive to the stimulus of light and respond by sending nerve impulses to the...

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Fast and sensitive detection of indels induced by precise gene targeting

Zhang Yang, Catharina Steentoft, Camilla Hauge, Lars Hansen, Allan Lind Thomsen, Francesco Niola, Malene B. Vester-Christensen, Morten Frödin, Henrik Clausen, Hans H. Wandall and Eric P. Bennett.

in Nucleic Acids Research

May 2015; p ublished online March 2015 .

Journal Article. Subjects: Research Methods in Life Sciences. 4194 words.

The nuclease-based gene editing tools are rapidly transforming capabilities for altering the genome of cells and organisms with great precision and in high throughput studies. A major...

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A highly sensitive selection method for directed evolution of homing endonucleases

Zhilei Chen and Huimin Zhao.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Research Methods in Life Sciences. 5592 words.

Homing endonucleases are enzymes that catalyze DNA sequence specific double-strand breaks and can significantly stimulate homologous recombination at these breaks. These enzymes have great...

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Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation

Srinivasan Yegnasubramanian, Xiaohui Lin, Michael C. Haffner, Angelo M. DeMarzo and William G. Nelson.

in Nucleic Acids Research

February 2006; p ublished online February 2006 .

Journal Article. Subjects: Research Methods in Life Sciences. 7638 words.

Hypermethylation of CpG island (CGI) sequences is a nearly universal somatic genome alteration in cancer. Rapid and sensitive detection of DNA hypermethylation would aid in cancer diagnosis...

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Sensitive and specific miRNA detection method using SplintR Ligase

Jingmin Jin, Sophie Vaud, Alexander M. Zhelkovsky, Janos Posfai and Larry A. McReynolds.

in Nucleic Acids Research

July 2016; p ublished online May 2016 .

Journal Article. Subjects: Research Methods in Life Sciences. 11022 words.

We describe a simple, specific and sensitive microRNA (miRNA) detection method that utilizes Chlorella virus DNA ligase (SplintR® Ligase). This two-step method involves ligation of adjacent...

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MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome

Hélène Thomassin, Clémence Kress and Thierry Grange.

in Nucleic Acids Research

November 2004; p ublished online November 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 6681 words.

Here we present MethylQuant, a novel method that allows accurate quantification of the methylation level of a specific cytosine within a complex genome. This method relies on the...

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DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA

Tongbo Wu, Wei Chen, Ziyu Yang, Haocheng Tan, Jiayu Wang, Xianjin Xiao, Mengyuan Li and Meiping Zhao.

in Nucleic Acids Research

February 2018; p ublished online November 2017 .

Journal Article. Subjects: Research Methods in Life Sciences. 7483 words.

Abstract

Sensitive detection of the single nucleotide variants in cell-free DNA (cfDNA) may provide great opportunity for minimally invasive diagnosis and...

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Enhanced transfection efficiency of a systemically delivered tumor‐targeting immunolipoplex by inclusion of a pH‐sensitive histidylated oligolysine peptide

Wei Yu, Kathleen F. Pirollo, Bin Yu, Antonina Rait, Laiman Xiang, Weiqun Huang, Qi Zhou, Gözen Ertem and Esther H. Chang.

in Nucleic Acids Research

March 2004; p ublished online March 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 7603 words.

Successful cancer gene therapy depends on the development of non‐toxic, efficient, tumor cell‐ specific systemic gene delivery systems. Our laboratory has developed a systemically...

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Sensitive and specific detection of K‐ras mutations in colon tumors by short oligonucleotide mass analysis

Matilde E. Lleonart, Santiago Ramón y Cajal, John D. Groopman and Marlin D. Friesen.

in Nucleic Acids Research

March 2004; p ublished online March 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 4969 words.

Short oligonucleotide mass analysis (SOMA) is a technique by which small sequences of mutated and wild‐type DNA, produced by PCR amplification and restriction digestion, are characterized...

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Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing

Anders Ståhlberg, Paul M. Krzyzanowski, Jennifer B. Jackson, Matthew Egyud, Lincoln Stein and Tony E. Godfrey.

in Nucleic Acids Research

June 2016; p ublished online April 2016 .

Journal Article. Subjects: Genetics and Genomics; Research Methods in Life Sciences. 4562 words.

Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be...

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Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes

Arun A. Tanpure and Seergazhi G. Srivatsan.

in Nucleic Acids Research

December 2015; p ublished online July 2015 .

Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 6990 words.

Development of probes that can discriminate G-quadruplex (GQ) structures and indentify efficient GQ binders on the basis of topology and nucleic acid type is highly desired to advance...

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The Significance of Meaning: Why Do Over 90% of Behavioral Neuroscience Results Fail to Translate to Humans, and What Can We Do to Fix It?

Joseph P. Garner.

in ILAR Journal

December 2014; p ublished online December 2014 .

Journal Article. Subjects: Research Methods in Life Sciences. 14492 words.

The vast majority of drugs entering human trials fail. This problem (called “attrition”) is widely recognized as a public health crisis, and has been discussed openly for the last two...

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Mining SAGE data allows large-scale, sensitive screening of antisense transcript expression

Ronan Quéré, Laurent Manchon, Mireille Lejeune, Oliver Clément, Fabien Pierrat, Béatrice Bonafoux, Thérèse Commes, David Piquemal and Jacques Marti.

in Nucleic Acids Research

November 2004; p ublished online November 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 3442 words.

As a growing number of complementary transcripts, susceptible to exert various regulatory functions, are being found in eukaryotes, high throughput analytical methods are needed to...

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Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications

Gergely Róna, Ildikó Scheer, Kinga Nagy, Hajnalka L. Pálinkás, Gergely Tihanyi, Máté Borsos, Angéla Békési and Beáta G. Vértessy.

in Nucleic Acids Research

February 2016; p ublished online October 2015 .

Journal Article. Subjects: Research Methods in Life Sciences. 9022 words.

The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important DNA element in diverse systems from specific phages to antibody...

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TELP, a sensitive and versatile library construction method for next-generation sequencing

Xu Peng, Jingyi Wu, Reinhard Brunmeir, Sun-Yee Kim, Qiongyi Zhang, Chunming Ding, Weiping Han, Wei Xie and Feng Xu.

in Nucleic Acids Research

March 2015; p ublished online September 2014 .

Journal Article. Subjects: Research Methods in Life Sciences. 8090 words.

Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated...

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Effect of lesions on the dynamics of DNA on the picosecond and nanosecond timescales using a polarity sensitive probe

Mark M. Somoza, Daniele Andreatta, Catherine J. Murphy, Robert S. Coleman and Mark A. Berg.

in Nucleic Acids Research

April 2004; p ublished online April 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 12098 words.

This paper explores the effects of structural modifications on the fast dynamics of DNA and the ability of time‐resolved Stokes shift spectroscopy to measure those changes. The...

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Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes

Kyle Cole, Vivi Truong, Dale Barone and Glenn McGall.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 5653 words.

Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses...

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Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes

Anna Válóczi, Csaba Hornyik, Nóra Varga, József Burgyán, Sakari Kauppinen and Zoltán Havelda.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Research Methods in Life Sciences. 4044 words.

We describe here a new method for highly efficient detection of microRNAs by northern blot analysis using LNA (locked nucleic acid)-modified oligonucleotides. In order to exploit the...

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Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking

Jan-Hendrik Spille, Tim P. Kaminski, Katharina Scherer, Jennifer S. Rinne, Alexander Heckel and Ulrich Kubitscheck.

in Nucleic Acids Research

January 2015; p ublished online November 2014 .

Journal Article. Subjects: Molecular and Cell Biology; Research Methods in Life Sciences. 8663 words.

Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light...

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A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

Kosuke Ishikawa, Yuta Kobayashi, Yutaro Wakabayashi, Shinya Watanabe and Kentaro Semba.

in Biology Methods and Protocols

P ublished online April 2018 .

Journal Article. Subjects: Biological Sciences; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology; Research Methods in Life Sciences. 5864 words.

Abstract

We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines...

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iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications

Alexis Autour, Eric Westhof and Michael Ryckelynck.

in Nucleic Acids Research

April 2016; p ublished online March 2016 .

Journal Article. Subjects: Research Methods in Life Sciences. 7251 words.

Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the...

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