sensitive
Overview page. Subjects: Medicine and Health.
adj. possessing the ability to respond to a stimulus. The cells of the retina, for example, are sensitive to the stimulus of light and respond by sending nerve impulses to the...
Fast and sensitive detection of indels induced by precise gene targeting
in Nucleic Acids Research
May 2015; p ublished online March 2015 .
Journal Article. Subjects: Research Methods in Life Sciences. 4194 words.
The nuclease-based gene editing tools are rapidly transforming capabilities for altering the genome of cells and organisms with great precision and in high throughput studies. A major...
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A highly sensitive selection method for directed evolution of homing endonucleases
in Nucleic Acids Research
January 2005; p ublished online January 2005 .
Journal Article. Subjects: Research Methods in Life Sciences. 5592 words.
Homing endonucleases are enzymes that catalyze DNA sequence specific double-strand breaks and can significantly stimulate homologous recombination at these breaks. These enzymes have great...
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Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation
in Nucleic Acids Research
February 2006; p ublished online February 2006 .
Journal Article. Subjects: Research Methods in Life Sciences. 7638 words.
Hypermethylation of CpG island (CGI) sequences is a nearly universal somatic genome alteration in cancer. Rapid and sensitive detection of DNA hypermethylation would aid in cancer diagnosis...
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Sensitive and specific miRNA detection method using SplintR Ligase
in Nucleic Acids Research
July 2016; p ublished online May 2016 .
Journal Article. Subjects: Research Methods in Life Sciences. 11022 words.
We describe a simple, specific and sensitive microRNA (miRNA) detection method that utilizes Chlorella virus DNA ligase (SplintR® Ligase). This two-step method involves ligation of adjacent...
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MethylQuant: a sensitive method for quantifying methylation of specific cytosines within the genome
in Nucleic Acids Research
November 2004; p ublished online November 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 6681 words.
Here we present MethylQuant, a novel method that allows accurate quantification of the methylation level of a specific cytosine within a complex genome. This method relies on the...
DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA
in Nucleic Acids Research
February 2018; p ublished online November 2017 .
Journal Article. Subjects: Research Methods in Life Sciences. 7483 words.
Abstract
Sensitive detection of the single nucleotide variants in cell-free DNA (cfDNA) may provide great opportunity for minimally invasive diagnosis and...
Enhanced transfection efficiency of a systemically delivered tumor‐targeting immunolipoplex by inclusion of a pH‐sensitive histidylated oligolysine peptide
in Nucleic Acids Research
March 2004; p ublished online March 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 7603 words.
Successful cancer gene therapy depends on the development of non‐toxic, efficient, tumor cell‐ specific systemic gene delivery systems. Our laboratory has developed a systemically...
Sensitive and specific detection of K‐ras mutations in colon tumors by short oligonucleotide mass analysis
in Nucleic Acids Research
March 2004; p ublished online March 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 4969 words.
Short oligonucleotide mass analysis (SOMA) is a technique by which small sequences of mutated and wild‐type DNA, produced by PCR amplification and restriction digestion, are characterized...
Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing
in Nucleic Acids Research
June 2016; p ublished online April 2016 .
Journal Article. Subjects: Genetics and Genomics; Research Methods in Life Sciences. 4562 words.
Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be...
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Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes
in Nucleic Acids Research
December 2015; p ublished online July 2015 .
Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 6990 words.
Development of probes that can discriminate G-quadruplex (GQ) structures and indentify efficient GQ binders on the basis of topology and nucleic acid type is highly desired to advance...
Mining SAGE data allows large-scale, sensitive screening of antisense transcript expression
in Nucleic Acids Research
November 2004; p ublished online November 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 3442 words.
As a growing number of complementary transcripts, susceptible to exert various regulatory functions, are being found in eukaryotes, high throughput analytical methods are needed to...
Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications
in Nucleic Acids Research
February 2016; p ublished online October 2015 .
Journal Article. Subjects: Research Methods in Life Sciences. 9022 words.
The role of uracil in genomic DNA has been recently re-evaluated. It is now widely accepted to be a physiologically important DNA element in diverse systems from specific phages to antibody...
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TELP, a sensitive and versatile library construction method for next-generation sequencing
in Nucleic Acids Research
March 2015; p ublished online September 2014 .
Journal Article. Subjects: Research Methods in Life Sciences. 8090 words.
Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated...
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Effect of lesions on the dynamics of DNA on the picosecond and nanosecond timescales using a polarity sensitive probe
in Nucleic Acids Research
April 2004; p ublished online April 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 12098 words.
This paper explores the effects of structural modifications on the fast dynamics of DNA and the ability of time‐resolved Stokes shift spectroscopy to measure those changes. The...
Direct labeling of RNA with multiple biotins allows sensitive expression profiling of acute leukemia class predictor genes
in Nucleic Acids Research
January 2004; p ublished online January 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 5653 words.
Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses...
Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes
in Nucleic Acids Research
January 2004; p ublished online January 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 4044 words.
We describe here a new method for highly efficient detection of microRNAs by northern blot analysis using LNA (locked nucleic acid)-modified oligonucleotides. In order to exploit the...
Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking
in Nucleic Acids Research
January 2015; p ublished online November 2014 .
Journal Article. Subjects: Molecular and Cell Biology; Research Methods in Life Sciences. 8663 words.
Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light...
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A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes
in Biology Methods and Protocols
P ublished online April 2018 .
Journal Article. Subjects: Biological Sciences; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology; Research Methods in Life Sciences. 5864 words.
Abstract
We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines...
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iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications
in Nucleic Acids Research
April 2016; p ublished online March 2016 .
Journal Article. Subjects: Research Methods in Life Sciences. 7251 words.
Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the...
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Multiplex mRNA assay using electrophoretic tags for high-throughput gene expression analysis
in Nucleic Acids Research
September 2004; p ublished online September 2004 .
Journal Article. Subjects: Research Methods in Life Sciences. 6560 words.
We describe a novel multiplexing technology using a library of small fluorescent molecules, termed eTag molecules, to code and quantify mRNA targets. eTag molecules, which have the same...
