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sensitive x Research Methods in Life Sciences x Molecular and Cell Biology x clear all

sensitive

Overview page. Subjects: Medicine and Health.

adj. possessing the ability to respond to a stimulus. The cells of the retina, for example, are sensitive to the stimulus of light and respond by sending nerve impulses to the...

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Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes

Arun A. Tanpure and Seergazhi G. Srivatsan.

in Nucleic Acids Research

December 2015; p ublished online July 2015 .

Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 6990 words.

Development of probes that can discriminate G-quadruplex (GQ) structures and indentify efficient GQ binders on the basis of topology and nucleic acid type is highly desired to advance...

Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking

Jan-Hendrik Spille, Tim P. Kaminski, Katharina Scherer, Jennifer S. Rinne, Alexander Heckel and Ulrich Kubitscheck.

in Nucleic Acids Research

January 2015; p ublished online November 2014 .

Journal Article. Subjects: Molecular and Cell Biology; Research Methods in Life Sciences. 8663 words.

Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light...

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A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

Kosuke Ishikawa, Yuta Kobayashi, Yutaro Wakabayashi, Shinya Watanabe and Kentaro Semba.

in Biology Methods and Protocols

P ublished online April 2018 .

Journal Article. Subjects: Biological Sciences; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology; Research Methods in Life Sciences. 5864 words.

Abstract

We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines...

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Simple and highly efficient BAC recombineering using galK selection

Søren Warming, Nina Costantino, Donald L. Court, Nancy A. Jenkins and Neal G. Copeland.

in Nucleic Acids Research

February 2005; p ublished online February 2005 .

Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 8230 words.

Recombineering allows DNA cloned in Escherichia coli to be modified via lambda (λ) Red-mediated homologous recombination, obviating the need for restriction enzymes and DNA ligases to modify...

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Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels

Shinya Sugimoto, Ken-ichi Arita-Morioka, Yoshimitsu Mizunoe, Kunitoshi Yamanaka and Teru Ogura.

in Nucleic Acids Research

August 2015; p ublished online April 2015 .

Journal Article. Subjects: Molecular and Cell Biology; Research Methods in Life Sciences. 6754 words.

The intrinsically stochastic dynamics of mRNA metabolism have important consequences on gene regulation and non-genetic cell-to-cell variability; however, no generally applicable methods...

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Mg2+-dependent conformational changes and product release during DNA-catalyzed RNA ligation monitored by Bimane fluorescence

Elisa Turriani, Claudia Höbartner and Thomas M. Jovin.

in Nucleic Acids Research

January 2015; p ublished online December 2014 .

Journal Article. Subjects: Research Methods in Life Sciences; Molecular and Cell Biology. 8980 words.

Among the deoxyribozymes catalyzing the ligation of two RNA substrates, 7S11 generates a branched RNA containing a 2′,5′-linkage. We have attached the small fluorogenic probe Bimane to the...

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The validity of the original EuroSCORE and EuroSCORE II in patients over the age of seventy

Michael Poullis, Mark Pullan, John Chalmers and Neeraj Mediratta.

in Interactive CardioVascular and Thoracic Surgery

February 2015; p ublished online October 2014 .

Journal Article. Subjects: Molecular Biology and Genetics; Molecular and Cell Biology; Cardiovascular Medicine; Research Methods in Life Sciences. 3077 words.

OBJECTIVES

EuroSCORE II, despite improving on the original EuroSCORE system, has not solved all the calibration and predictability issues. We investigated...