Journal Article

An Anti-Chitinase Malaria Transmission–Blocking Single-Chain Antibody as an Effector Molecule for Creating a Plasmodium falciparum–Refractory Mosquito

Fengwu Li, Kailash P. Patra and Joseph M. Vinetz

in The Journal of Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 192, issue 5, pages 878-887
Published in print September 2005 | ISSN: 0022-1899
Published online September 2005 | e-ISSN: 1537-6613 | DOI: https://dx.doi.org/10.1086/432552
An Anti-Chitinase Malaria Transmission–Blocking Single-Chain Antibody as an Effector Molecule for Creating a Plasmodium falciparum–Refractory Mosquito

More Like This

Show all results sharing these subjects:

  • Infectious Diseases
  • Immunology
  • Public Health and Epidemiology
  • Microbiology

GO

Show Summary Details

Preview

Indirect evidence has suggested the existence of a second chitinase gene, PgCHT2 in the avian malaria parasite Plasmodium gallinaceum. We have now identified PgCHT2 as the orthologue of the P. falciparum chitinase gene PfCHT1 a malaria transmission–blocking target. Computational phylogenetic evidence and biochemical and cell biological functional data support the hypothesis that an avian-related Plasmodium species was the ancestor of both P. falciparum and P. reichenowi and this single lineage gave rise to another lineage of malaria parasites, including P. vivax, P. knowlesi, P. berghei, P. yoelii and P. chabaudi. A recombinant PfCHT1/PgCHT2-neutralizing single-chain antibody significantly reduced P. falciparum and P. gallinaceum parasite transmission to mosquitoes. This single-chain antibody is the first anti–P. falciparum effector molecule to be validated for making a malaria transmission–refractory transgenic Anopheles species mosquito. P. gallinaceum is a relevant animal model that facilitates a mechanistic understanding of P. falciparum invasion of the mosquito midgut

Journal Article.  5256 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content. subscribe or login to access all content.