Journal Article

<i>In vitro</i> Bulb Development in Shallot (<i>Allium cepa</i> L. Aggregatum Group): Effects of Anti‐gibberellins, Sucrose and Light

F. LE GUEN‐LE SAOS, A. HOURMANT, F. ESNAULT and J. E. CHAUVIN

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 89, issue 4, pages 419-425
Published in print April 2002 | ISSN: 0305-7364
Published online April 2002 | e-ISSN: 1095-8290 | DOI: https://dx.doi.org/10.1093/aob/mcf063
In vitro Bulb Development in Shallot (Allium cepa L. Aggregatum Group): Effects of Anti‐gibberellins, Sucrose and Light

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Bulbing was studied in shallot plants cultured in vitro. Bulbing occurred under a 16 h photoperiod with fluorescent + incandescent light and 30–50 g l–1 sucrose in the culture medium. Exogenous gibberellin (10 µm GA3) inhibited leaf and root growth and bulbing. When added to the medium at a concentration of 10 µm, three inhibitors of gibberellin biosynthesis (ancymidol, flurprimidol and paclobutrazol) promoted bulb formation and the percentage of bulbing. When ancymidol was used in combination with GA3, it did not reverse the effect of GA3 applied alone. Under treatments with 30–70 g l–1 sucrose, bulbing ratios greater than those found in control plants were achieved by addition of ancymidol, and bulb fresh weight was increased in the same way. Ancymidol caused a 66 % decrease in sucrose content in leaf bases but greatly increased the glucose, fructose and fructan contents. The increase in fructan content by ancymidol could result from the three‐fold rise in total [14C]sucrose uptake per plant from the culture medium associated with a marked increase in leaf base labelling at the expense of root labelling. The possible role of ancymidol is discussed and evidence supports a major regulatory role for gibberellins in bulbing.

Keywords: Key words: Shallot, Allium cepa L. Aggregatum Group, in vitro bulbing, ancymidol, light quality, sucrose, carbohydrate content.

Journal Article.  4336 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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