Journal Article

Multivariate exploratory tools for microarray data analysis

Aniko Szabo, Kenneth Boucher, David Jones, Alexander D. Tsodikov, Lev B. Klebanov and Andrei Y. Yakovlev

in Biostatistics

Volume 4, issue 4, pages 555-567
Published in print October 2003 | ISSN: 1465-4644
Published online October 2003 | e-ISSN: 1468-4357 | DOI:
Multivariate exploratory tools for microarray data analysis

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The ultimate success of microarray technology in basic and applied biological sciences depends critically on the development of statistical methods for gene expression data analysis. The most widely used tests for differential expression of genes are essentially univariate. Such tests disregard the multidimensional structure of microarray data. Multivariate methods are needed to utilize the information hidden in gene interactions and hence to provide more powerful and biologically meaningful methods for finding subsets of differentially expressed genes. The objective of this paper is to develop methods of multidimensional search for biologically significant genes, considering expression signals as mutually dependent random variables. To attain these ends, we consider the utility of a pertinent distance between random vectors and its empirical counterpart constructed from gene expression data. The distance furnishes exploratory procedures aimed at finding a target subset of differentially expressed genes. To determine the size of the target subset, we resort to successive elimination of smaller subsets resulting from each step of a random search algorithm based on maximization of the proposed distance. Different stopping rules associated with this procedure are evaluated. The usefulness of the proposed approach is illustrated with an application to the analysis of two sets of gene expression data.

Keywords: Cross‐validation; Differential expression; Permutation test; Probability distance; Random search; Sets of genes

Journal Article.  0 words. 

Subjects: Probability and Statistics

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