Journal Article

Periodic Gene Expression Patterns during the Highly Synchronized Cell Nucleus and Organelle Division Cycles in the Unicellular Red Alga Cyanidioschyzon merolae

Takayuki Fujiwara, Osami Misumi, Kousuke Tashiro, Yamato Yoshida, Keiji Nishida, Fumi Yagisawa, Sousuke Imamura, Masaki Yoshida, Toshiyuki Mori, Kan Tanaka, Haruko Kuroiwa and Tsuneyoshi Kuroiwa

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 16, issue 1, pages 59-72
Published in print February 2009 | ISSN: 1340-2838
Published online January 2009 | e-ISSN: 1756-1663 | DOI: https://dx.doi.org/10.1093/dnares/dsn032

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Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.

Keywords: cell cycle; microarray; mitochondria–plastid division genes; organelle division genes; Cyanidioschyzon merolae

Journal Article.  4322 words.  Illustrated.

Subjects: Genetics and Genomics

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