Journal Article

Stabilization of Dry Mammalian Cells: Lessons from Nature1

John H. Crowe, Lois M. Crowe, Willem F. Wolkers, Ann E. Oliver, Xiaocui Ma, Joong-Hyuck Auh, Minke Tang, Shijun Zhu, Jeffrey Norris and Fern Tablin

in Integrative and Comparative Biology

Published on behalf of The Society for Integrative and Comparative Biology

Volume 45, issue 5, pages 810-820
Published in print November 2005 | ISSN: 1540-7063
Published online November 2005 | e-ISSN: 1557-7023 | DOI: https://dx.doi.org/10.1093/icb/45.5.810
Stabilization of Dry Mammalian Cells: Lessons from Nature1

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The Center for Biostabilization at UC Davis is attempting to stabilize mammalian cells in the dry state. We review here some of the lessons from nature that we have been applying to this enterprise, including the use of trehalose, a disaccharide found at high concentrations in many anhydrobiotic organisms, to stabilize biological structures, both in vitro and in vivo. Trehalose has useful properties for this purpose and in at least in one case—human blood platelets—introducing this sugar may be sufficient to achieve useful stabilization. Nucleated cells, however, are stabilized by trehalose only during the initial stages of dehydration. Introduction of a stress protein obtained from an anhydrobiotic organism, Artemia, improves the stability markedly, both during the dehydration event and following rehydration. Thus, it appears that the stabilization will require multiple adaptations, many of which we propose to apply from studies on anhydrobiosis.

Journal Article.  8416 words.  Illustrated.

Subjects: Biological Sciences

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