Journal Article

Analysis of chromosome translocation frequency after a single CT scan in adults

Yu Abe, Tomisato Miura, Mitsuaki A. Yoshida, Risa Ujiie, Yumiko Kurosu, Nagisa Kato, Atsushi Katafuchi, Naohiro Tsuyama, Fumihiko Kawamura, Takashi Ohba, Tomoko Inamasu, Fumio Shishido, Hideyoshi Noji, Kazuei Ogawa, Hiroshi Yokouchi, Kenya Kanazawa, Takashi Ishida, Satoshi Muto, Jun Ohsugi, Hiroyuki Suzuki, Tetsuo Ishikawa, Kenji Kamiya and Akira Sakai

in Journal of Radiation Research

Volume 57, issue 3, pages 220-226
Published in print June 2016 | ISSN: 0449-3060
Published online June 2016 | e-ISSN: 1349-9157 | DOI: https://dx.doi.org/10.1093/jrr/rrv090

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  • Clinical Genetics
  • Molecular Biology and Genetics
  • Epidemiology
  • Radiology
  • Nuclear Chemistry, Photochemistry, and Radiation

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We recently reported an increase in dicentric chromosome (DIC) formation after a single computed tomography (CT) scan (5.78–60.27 mSv: mean 24.24 mSv) and we recommended analysis of 2000 metaphase cells stained with Giemsa and centromere-FISH for dicentric chromosome assay (DCA) in cases of low-dose radiation exposure. In the present study, we analyzed the frequency of chromosome translocations using stored Carnoy's-fixed lymphocyte specimens from the previous study; these specimens were from 12 patients who were subject to chromosome painting of Chromosomes 1, 2 and 4. Chromosomes 1, 2 and 4 were analyzed in ∼5000 cells, which is equivalent to the whole-genome analysis of almost 2000 cells. The frequency of chromosome translocation was higher than the number of DICs formed, both before and after CT scanning. The frequency of chromosome translocations tended to be higher, but not significantly higher, in patients with a treatment history compared with patients without such a history. However, in contrast to the results for DIC formation, the frequency of translocations detected before and after the CT scan did not differ significantly. Therefore, analysis of chromosome translocation may not be a suitable assay for detecting chromosome aberrations in cases of low-dose radiation exposure from a CT scan. A significant increase in the frequency of chromosome translocations was not likely to be detected due to the high baseline before the CT scan; the high and variable frequency of translocations was probably due to multiple confounding factors in adults.

Keywords: dicentric chromosome; translocated chromosome; chromosome painting; low-dose radiation exposure; confounding factors

Journal Article.  4220 words.  Illustrated.

Subjects: Clinical Genetics ; Molecular Biology and Genetics ; Epidemiology ; Radiology ; Nuclear Chemistry, Photochemistry, and Radiation

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