Journal Article

Effect of radiation-induced endothelial cell injury on platelet regeneration by megakaryocytes

Fang Chen, Mingqiang Shen, Dongfeng Zeng, Cheng Wang, Song Wang, Shilei Chen, Yong Tang, Mengjia Hu, Mo Chen, Yongping Su, Xinze Ran, Yang Xu and Junping Wang

in Journal of Radiation Research

Volume 58, issue 4, pages 456-463
Published in print July 2017 | ISSN: 0449-3060
Published online April 2017 | e-ISSN: 1349-9157 | DOI: https://dx.doi.org/10.1093/jrr/rrx015
Effect of radiation-induced endothelial cell injury on platelet regeneration by megakaryocytes

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  • Clinical Genetics
  • Molecular Biology and Genetics
  • Epidemiology
  • Radiology
  • Nuclear Chemistry, Photochemistry, and Radiation

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Abstract

Thrombocytopenia is an important cause of hemorrhage and death after radiation injury, but the pathogenesis of radiation-induced thrombocytopenia has not been fully characterized. Here, we investigated the influence of radiation-induced endothelial cell injury on platelet regeneration. We found that human umbilical vein endothelial cells (HUVECs) underwent a high rate of apoptosis, accompanied by a significant reduction in the expression of vascular endothelial growth factor (VEGF) at 96 h after radiation. Subsequent investigations revealed that radiation injury lowered the ability of HUVECs to attract migrating megakaryocytes (MKs). Moreover, the adhesion of MKs to HUVECs was markedly reduced when HUVECs were exposed to radiation, accompanied by a decreased production of platelets by MKs. In vivo study showed that VEGF treatment significantly promoted the migration of MKs into the vascular niche and accelerated platelet recovery in irradiated mice. Our studies demonstrate that endothelial cell injury contributes to the slow recovery of platelets after radiation, which provides a deeper insight into the pathogenesis of thrombocytopenia induced by radiation.

Keywords: radiation; endothelial cell; megakaryocyte; platelet; VEGF

Journal Article.  3818 words.  Illustrated.

Subjects: Clinical Genetics ; Molecular Biology and Genetics ; Epidemiology ; Radiology ; Nuclear Chemistry, Photochemistry, and Radiation

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