Journal Article

Identification of new minisatellites loci in Arabidopsis thaliana

S. Tourmente, A. Lazreg, J. Lafleuriel, S. Tutois, C. Cuvillier, M.C. Espagnol and G. Picard

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 49, issue 318, pages 21-25
Published in print January 1998 | ISSN: 0022-0957
Published online January 1998 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/49.318.21
Identification of new minisatellites loci in Arabidopsis thaliana

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In order to characterize new GC-rich minisatellites present in the Arabidopsis thaliana genome, a genomic library was screened at low stringency with a probe containing nine repeated-units of a minisatellite (CMs1) previously identified. Both minisatellites and minisatellite-like elements were identified. The minisatellites, with a tandemly-repeated structure, all contain the Arabidopsis thaliana-core sequence previously defined (Tourmente et al., 1994). Both minisatellite and minisatellite-like sequences occur in the Arabidopsis genome in low copy and are weakly polymorphic between ecotypes. The genetic mapping of these markers has shown that they are dispersed on the genome. YACs clones of the CIC library carrying these minisatellites and minisatellite-like sequences were identified.

Keywords: Arabidopsis thaliana; minisatellites; polymorphism

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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