Journal Article

The gene geranylgeranyl reductase of peach (Prunus persica [L.] Batsch) is regulated during leaf development and responds differentially to distinct stress factors

Donato Giannino, Emiliano Condello, Leonardo Bruno, Giulio Testone, Andrea Tartarini, Radiana Cozza, Anna Maria Innocenti, Maria Beatrice Bitonti and Domenico Mariotti

in Journal of Experimental Botany

Published on behalf of Society for Experimental Biology

Volume 55, issue 405, pages 2063-2073
Published in print September 2004 | ISSN: 0022-0957
Published online July 2004 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/erh217
The gene geranylgeranyl reductase of peach (Prunus persica [L.] Batsch) is regulated during leaf development and responds differentially to distinct stress factors

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Plant geranylgeranyl hydrogenase (CHL P) reduces free geranylgeranyl diphosphate to phytil diphosphate, which provides the side chain to chlorophylls, tocopherols, and plastoquinones. In peach, the single copy gene (PpCHL P) encodes a deduced product of 51.68 kDa, which harbours a transit peptide for cytoplasm-to-chloroplast transport and a nicotinamide binding domain. The PpCHL P message was abundant in chlorophyll-containing tissues and flower organs, but barely detected in the roots and mesocarp of ripening fruits, suggesting that transcription was related to plastid types and maturation. The message was not revealed in shoot apical meristems, but spread thoroughly in leaf cells during the early stages and was located mainly in the palisade of mature leaves, which exhibited higher transcript levels than young ones. Hence, the transcription of PpCHL P was likely to be regulated during leaf development. Gene expression was monitored in leaves responding to natural dark, cold, wounding, stress by imposed darkening, and during the curl disease. Transcription was stimulated by light, but repressed by dark and cold stress. In darkened leaves, the PpCHL P message was augmented concomitantly with that of CATALASE. In wounded leaves, the message decreased, but recovered rapidly, whereas in curled leaves, a reduction in gene expression was related to leaf damage intensity. However, transcript signals increased locally both in cells mechanically wounded by a needle and in those naturally injured by the pathogenic fungus Taphrina deformans. These data suggest that PpCHL P expression was regulated by photosynthetic activity and was possibly involved in the defence response.

Keywords: Geranylgeranyl reductase; leaf development; peach; Prunus persica L.; regulation; stress response

Journal Article.  6446 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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