Journal Article

Rab geranylgeranyl transferase β subunit is essential for male fertility and tip growth in <i>Arabidopsis</i>

Malgorzata Gutkowska, Marta Wnuk, Julita Nowakowska, Malgorzata Lichocka, Michal M. Stronkowski and Ewa Swiezewska

in Journal of Experimental Botany

Volume 66, issue 1, pages 213-224
Published in print January 2015 | ISSN: 0022-0957
Published online October 2014 | e-ISSN: 1460-2431 | DOI: https://dx.doi.org/10.1093/jxb/eru412

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Rab proteins, key players in vesicular transport in all eukaryotic cells, are post-translationally modified by lipid moieties. Two geranylgeranyl groups are attached to the Rab protein by the heterodimeric enzyme Rab geranylgeranyl transferase (RGT) αβ. Partial impairment in this enzyme activity in Arabidopsis, by disruption of the AtRGTB1 gene, is known to influence plant stature and disturb gravitropic and light responses. Here it is shown that mutations in each of the RGTB genes cause a tip growth defect, visible as root hair and pollen tube deformations. Moreover, FM 1–43 styryl dye endocytosis and recycling are affected in the mutant root hairs. Finally, it is demonstrated that the double mutant, with both AtRGTB genes disrupted, is non-viable due to absolute male sterility. Doubly mutated pollen is shrunken, has an abnormal exine structure, and shows strong disorganization of internal membranes, particularly of the endoplasmic reticulum system.

Keywords: Arabidopsis; male fertility; pollen; pollen tube; protein prenylation; Rab geranylgeranyl transferase; Rab protein; root hair; tip growth.

Journal Article.  7608 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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