Journal Article

Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency

K. Sermon, P. Henderix, W. Lissens, A.De Vos, M. Vandervorst, A. Vanderfaeillie, E. Vamos, A.Van Steirteghem and I. Liebaers

in MHR: Basic science of reproductive medicine

Published on behalf of European Society of Human Reproduction and Embryology

Volume 6, issue 12, pages 1165-1168
Published in print December 2000 | ISSN: 1360-9947
Published online December 2000 | e-ISSN: 1460-2407 | DOI:
Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency

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Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of life with recurrent episodes of hypoketotic hypoglycaemia and lethargy leading to death in ~25% of the cases. One mutation (c985A→G) accounts for ~90% of the carrier chromosomes. We developed a preimplantation genetic diagnosis (PGD) strategy for MCAD for a couple who had already lost two affected children. When tested on heterozygous lymphoblasts, the amplification efficiency was 67 out of 71 (94%) and the allele drop-out rate was 0 out of 67. The patient became pregnant after one PGD cycle during which two embryos were replaced. The twin pregnancy was checked by chorionic villus sampling (CVS) and was shown to be unaffected. The twins have been born and are healthy.

Keywords: medium-chain acyl-CoA dehydrogenase deficiency; preimplantation genetic diagnosis; single-cell PCR

Journal Article.  3054 words.  Illustrated.

Subjects: Reproductive Medicine

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