Journal Article

Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant

Bin Kang, Hao Wang, Kyoung Hee Nam, Jiayang Li and Jianming Li

in Molecular Plant

Published on behalf of IPPE, SIBS, CAS and Chinese Society for Plant Biology (CSPB)

Volume 3, issue 1, pages 260-268
Published in print January 2010 | ISSN: 1674-2052
Published online December 2009 | e-ISSN: 1752-9867 | DOI: https://dx.doi.org/10.1093/mp/ssp099
Activation-Tagged Suppressors of a Weak Brassinosteroid Receptor Mutant

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Brassinosteroids (BRs) are important plant hormones that act synergistically with auxin to regulate a variety of plant developmental and physiological processes. In the past decade, genetic and biochemical studies have revealed a linear signaling pathway that relies on protein phosphorylation to transmit the BR signal into the nucleus, altering expression of hundreds of genes to promote plant growth. We conducted an activation-tagging based suppressor screen to look for Arabidopsis genes that, when overexpressed by inserted 35S enhancer elements, could suppress the dwarf phenotype of a weak BR receptor mutant bri1-301. This screen identified a total of six dominant activation-tagged bri1 suppressors (atbs-Ds). Using a plasmid rescue approach, we discovered that the bri1-301 suppression effect in four atbs-D mutants (atbs3-D to atbs6-D) was caused by overexpression of a YUCCA gene thought to be involved in tryptophan-dependent auxin biosynthesis. Interestingly, the three activation-tagged YUCCA genes belong to the YUCCA IIA subfamily that includes two other members out of 11 known Arabidopsis YUCCA genes. In addition, our molecular studies revealed a T-DNA insertion near a basic helix-loop-helix gene in atbs1-D and a T-DNA insertion in a region carrying a BR biosynthetic gene in atbs2-D. Further studies of these atbs-D mutants could lead to better understanding of the BR signaling process and the BR–auxin interaction.

Keywords: Brassinosteroid; BRI1; bri1-301; auxin; YUCCA

Journal Article.  5086 words.  Illustrated.

Subjects: Molecular and Cell Biology ; Plant Sciences and Forestry

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