Journal Article

The DNA Supercoiling Architecture Induced by the Transcription Factor xUBF Requires Three of Its Five HMG-Boxes

V. Y. Stefanovsky, D. P. Bazett-Jones, G. Pelletier and T. Moss

in Nucleic Acids Research

Volume 24, issue 16, pages 3208-3215
Published in print August 1996 | ISSN: 0305-1048
Published online August 1996 | e-ISSN: 1362-4962 | DOI: https://dx.doi.org/10.1093/nar/24.16.3208
The DNA Supercoiling Architecture Induced by the Transcription Factor xUBF Requires Three of Its Five HMG-Boxes

More Like This

Show all results sharing these subjects:

  • Chemistry
  • Biochemistry
  • Bioinformatics and Computational Biology
  • Genetics and Genomics
  • Molecular and Cell Biology

GO

Show Summary Details

Preview

The formation of a near complete loop of DNA is a striking property of the architectural HMG-box factor xUBF. Here we show that DNA looping only requires a dimer of Nbox13, a C-terminal truncation mutant of xUBF containing just HMG-boxes 1–3. This segment of xUBF corresponds to that minimally required for activation of polymerase I transcription and is sufficient to generate the major characteristics of the footprint given by intact xUBF. Stepwise reduction in the number of HMG-boxes to less than three significantly diminishes DNA bending and provides an estimate of bend angle for each HMG-box. Together the data indicate that a 350 ± 16° loop in 142 ± 30 bp of DNA can be induced by binding of the six HMG-boxes in an Nbox13 dimer and that DNA looping is probably achieved by six in-phase bends. The positioning of each HMG-box on the DNA does not predominantly involve DNA sequence recognition and is thus an intrinsic property of xUBF.

Journal Article.  4240 words.  Illustrated.

Subjects: Chemistry ; Biochemistry ; Bioinformatics and Computational Biology ; Genetics and Genomics ; Molecular and Cell Biology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content. subscribe or login to access all content.